“蒂达”类藏药川西獐牙菜的HPLC指纹图谱研究  

Fingerprints of commonly used“Dida”Tibetan medicine Swertia mussotii Franch by HPLC

作　　者：杨增亮 刘学良 苏媛 李维业 刘安平 王开祥 刘海青 鲁尚静 刘文平 YANG Zeng-liang;LIU Xue-liang;SU Yuan;LI Wei-ye;LIU An-ping;WANG Kai-xiang;LIU Hai-qing;LU Shang-jing;LIU Wen-ping(Xining Center for Food and Drug Inspection&Testing,Xining 810007;Qinghai Drug Evaluation and Verification Center,Xining 810007;Qinghai Sanpu Pharmaceutical Co.,Ltd.,Xining 810007)

机构地区：[1]西宁市食品药品检验检测中心,西宁810007 [2]青海省药品审评核查中心,西宁810007 [3]青海三普药业有限公司,西宁810007

出　　处：《中南药学》2023年第6期1625-1629,共5页Central South Pharmacy

基　　金：青海省基础研究项目(No.2020-ZJ-747)。

摘　　要：目的建立“蒂达”类藏药川西獐牙菜的HPLC指纹图谱,并结合化学计量学分析进行常用“蒂达”类藏药川西獐牙菜药材的质量分析。方法采用Waters SunFire C18柱(4.6mm×250 mm,5μm),以甲醇-水(含0.05%磷酸)为流动相,进行梯度洗脱,流速1.0mL·min^(-1),检测波长254 nm,柱温30℃。对不同来源和不同批次的川西獐牙菜进行指纹图谱测定,并通过化学计量学系统解决方案软件(ChemPattern 2017版)对结果进行处理分析。结果14批次川西獐牙菜药材与生成对照指纹图谱的相似度在0.923~1.000,具有较好的一致性。标定了22个共有峰,并对其中9个峰进行了指认,分别为马钱苷酸、獐芽菜苦苷、龙胆苦苷、当药苷、芒果苷、异荭草素、当药醇苷、1,8-二羟基-3,7-二甲氧基酮、8-O-β-D-吡喃葡萄糖-1,3,5-三羟基酮。主成分分析提取出3个主成分进行综合评价,可以反映样品数据中的绝大部分信息。聚类分析将样品分为2类,采集于青海地区的样品S01、S02、S04~S13聚为一组,采集于四川、西藏地区的样品S03、S14聚为一组。结论该方法科学、准确且简便易行,方法精密度、稳定性和重复性良好,专属性强,可以作为常用“蒂达”类藏药川西獐牙菜的质量控制方法。Objective To establish the chromatographic fingerprints of commonly used“Dida”Tibetan medicine swertia in Swertia mussotii Franch by HPLC and analyze its quality with chemometrics.Methods Waters SunFire C18 column(4.6 mm×250 mm,5μm)was used with methanol-0.05%phosphoric acid solution as the mobile phase.The flow rate was 1.0 mL·min^(-1) with gradient elution.The detection wavelength was 254 nm and the column temperature was 30℃.The fingerprints of Swertia mussotii Franch from different sources and batches were determined,the results were analyzed through chemometrics system solution software(ChemPattern 2017).Results The similarity between the 14 batches of Swertia mussotii Franch and the generated reference fingerprint was 0.923~1.000 with good consistency.Totally 22 common peaks were calibrated and 9 of them were identified as loganin acid,swertiamarin,gentiopicroside,sweroside,mangiferin,isoorientin,swertianolin,1,8-dihydroxy-3,7-dimethoxy-xanthone,and 8-O-β-D-glucopyranosyl-1,3,5-trihydroxy-xanthone.Principal component analysis was used to extract three principal components for comprehensive evaluation,which reflected most information in the sample data.Cluster analysis divided the samples into two categories.Among the 14 batches of samples,S01,S02,and S04~S13 collected in Qinghai belonged to one group,while S03 and S14 collected in Sichuan and Tibet were another group.Conclusion The method is scientific,accurate and easy to operate with good precision,stability,repeatability and specificity,which can be used for the quality control of commonly used“Dida”Tibetan medicine Swertia mussotii Franch.

关 键 词：川西獐牙菜 HPLC指纹图谱 质量控制 化学计量学分析 

分 类 号：R284.1[医药卫生—中药学]