甲醇对牛γ-球蛋白液-液相分离的调控研究  

Study on theregulation of methanol on liquid-liquid phase separation of Bovineγ-globulin

作　　者：李晋 郑寓 王艳伟[1] 杨光参[1] LI Jin;ZHENG Yu;WANG Yanwei;YANG Guangcan(School of Mathematics and Physics,Wenzhou University,Wenzhou 325035,China)

机构地区：[1]温州大学数理学院,浙江温州325035

出　　处：《齐鲁工业大学学报》2023年第3期62-68,共7页Journal of Qilu University of Technology

基　　金：国家自然科学基金(12074289,11574232)。

摘　　要：主要围绕聚乙二醇-1000(PEG-1000)和甲醇对牛γ-球蛋白(Bovineγ-Globulin)液-液相分离的调控展开。首先观察PEG-1000对牛γ-球蛋白液-液相分离的影响,绘制其二维相图。其次在以上体系中加入甲醇,随着甲醇质量浓度从10 mg·mL^(-1)提升至100 mg·mL^(-1),牛γ-球蛋白混合液的状态发生明显变化;当甲醇质量浓度处于50 mg·mL^(-1)时,与未添加甲醇相比,相分离区域下移扩大。最后在含有甲醇的体系中再加入疏水屏蔽剂1,6-己二醇和静电作用屏蔽剂6-氨基乙酸进行调控,发现液-液相分离被有效抑制。同时结合微量紫外分光光度计测量溶液的吸光度变化,与上述结果一致,由此得出甲醇对液-液相分离的驱动是疏水和静电协同作用的结果。This study focuses on the regulation of PEG-1000 and methanol on the liquid-liquid phase separation of bovineγ-globulin.Firstly,the effect of PEG-1000 on the liquid-liquid phase separation of bovineγ-globulin was observed,and its two-dimensional phase diagram was drawn.Secondly,methanol was added to the above system,and it was found that the state of bovineγ-globulin mixture changed significantly with the increase of methanol concentration from 10 mg·mL^(-1) to 100 mg·mL^(-1).When the methanol concentration is at 50 mg·mL^(-1),the phase separation region is enlarged compared with that without methanol.Finally,hydrophobic shielding agent 1,6-hexanediol and electrostatic shielding agent 6-aminoacetic acid were added to the system containing methanol,and it was found that liquid-liquid phase separation was effectively inhibited.At the same time,combined with the absorbance change of the solution measured by the micro ultraviolet spectrophotometer,which is consistent with the above results,it can be concluded that the driving of methanol on liquid-liquid phase separation is the result of the synergistic effect of hydrophobicity and static electricity.

关 键 词：液-液相分离 牛 γ -球蛋白 甲醇 聚乙二醇 

分 类 号：Q27[生物学—细胞生物学]