牛4种主要传染病的检测与分析  

Detection and Analysis of Four Major Infectious Diseases in Cattle

作　　者：谢丽华[1] 植枝林 戴光文 梁锦钊 XIE Li-hua;ZHI Zhi-lin;DAI Guang-wen;LIANG Jin-zhao(Wuzhou Animal Disease Control and Prevention,Wuzhou,Guangxi 543002;Cenxi Animal Disease Control and Prevention Center,Cenxi,Guangxi 543200;Wuzhou Agricultural Comprehensive Administrative Law Enforcement Detachment,Wuzhou,Guangxi 543002)

机构地区：[1]梧州市动物疫病预防控制中心,广西梧州543002 [2]岑溪市动物疫病预防控制中心,广西岑溪543200 [3]梧州市农业综合行政执法支队,广西梧州543002

出　　处：《中国牛业科学》2023年第2期43-45,57,共4页China Cattle Science

基　　金：2021年梧州市科学研究与技术开发计划“牛羊繁育扩产和疫病防控关键技术研究与应用”项目(202102020)。

摘　　要：[目的]旨在研究口蹄疫、布鲁氏菌病、牛结核病、牛结节性皮肤病等4种牛主要传染病的免疫效果和流行情况。[方法]采用疫苗对口蹄疫、牛结节性皮肤病免疫,针对性使用荧光PCR、ELISA方法以及虎红平板凝集试验、皮内变态反应试验对上述4种牛病进行检测。[结果]免疫牛口蹄疫10.21万头次,检测血清1534份和组织样品233份,口蹄疫免疫抗体合格率94.33%,口蹄疫病毒为阴性。使用山羊痘疫苗免疫牛结节性皮肤病2.89万头次,检测口鼻拭子/抗凝样品200份,牛结节性皮肤病病毒为阴性。监测牛布鲁氏菌病血清2380份,阳性率为0.17%。监测牛结核病1291头,阳性率为0.23%。[结论]口蹄疫、牛结节性皮肤病免疫效果良好,达到阻断病毒感染和传播目的。布鲁氏菌病、牛结核病总体上分别达到控制标准和净化标准,但“两病”仍有零星发生,需要加强牛只调运监管检疫。[Objective]The purpose is to investigate the immune effect and prevalence of four major cattle infectious diseases of foot-and-mouth disease(FMD),brucellosis,bovine tuberculosis(bTB)and lumpy skin disease(LSD).[Method]The vaccines were used to immunize against foot-and-mouth disease and lumpy skin disease.Fluorescent PCR,ELISA,Rose-Bengal plate agglutination examination and PPD intradermic allergy test were used to detect the above four bovine diseases.[Result]A total of 102100 cattle were immunized against FMD.1534 serum samples and 233 tissue samples were tested.The qualified rate of FMD antibody was 94.33%,and the FMD virus was negative.A total of 28900 cattle was immunized with goat pox vaccine against LSD,and 200 oral and nasal swabs and anticoagulant samples were tested,which were negative for LSDV.2380 serum samples of bovine brucellosis were monitored,and the positive rate was 0.17%.1291 cattle were monitored for tuberculosis,and the positive rate was 0.23%.[Conclusion]The results showed that the immune effect of foot-and-mouth disease and lumpy skin disease was better,and the purpose of blocking virus infection and transmission was achieved.Brucellosis and bovine tuberculosis generally meet the control and purification standards,respectively.However,the diseases still occur sporadically,and it is necessary to strengthen the supervision and quarantine of cattle transportation.

关 键 词：口蹄疫 布鲁氏菌病 结核病 牛结节性皮肤病 检测与分析 

分 类 号：S851.3[农业科学—预防兽医学]