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作 者:李尹琳 毕铭 夏方山 王聪聪 曾佳 LI Yinlin;BI Ming;XIA Fangshan;WANG Congcong;ZENG Jia(College of Grassland Science,Shanxi Agricultural University,Taigu030801,China)
出 处:《畜牧与饲料科学》2023年第3期83-88,共6页Animal Husbandry and Feed Science
基 金:中央引导地方科技发展资金项目(YDZJSX2022C 011);山西省重点研发计划项目(201903D221091,202102140601006);山西农业大学“十四五”生物育种工程项目(YZGC134);内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)。
摘 要:[目的]探究外源氮引发对白羊草(Bothriochloa ischaemum)种子萌发特性的影响。[方法]以白羊草种子为试验材料,分别采用不同浓度(0、70、140、280、560、1120 mmol/L)的NH_(4)NO_(3)溶液引发0(CK)、3、6、9、12 h后,分析种子发芽率、发芽指数、平均发芽时间及幼苗活力指数的变化,揭示白羊草种子萌发对外源氮引发的响应。[结果]氮浓度、引发时间及二者的交互作用对白羊草种子发芽率、发芽指数、平均发芽时间及幼苗活力指数均具有极显著(P<0.01)的影响。白羊草种子发芽率、发芽指数和幼苗活力指数在NH_(4)NO_(3)溶液浓度为0~140 mmol/L时呈上升趋势,在NH_(4)NO_(3)浓度为280~1120 mmol/L时呈下降趋势,而平均发芽时间的变化趋势与之相反。[结论]白羊草种子萌发能力在低浓度(≤140 mmol/L)NH_(4)NO_(3)溶液引发时升高,在高浓度(≥280 mmol/L)NH_(4)NO_(3)溶液引发时降低。浓度为140 mmol/L引发6 h是生产实践中应用NH_(4)NO_(3)溶液对白羊草种子进行氮引发的最佳处理。[Objective]This study aimed to explore the effects of exogenous nitrogen priming on seed germination characteristics of Bothriochloa ischaemum.[Method]The Bothriochloa ischaemum seeds were used as experimental materials.After priming with different concentrations(0,70,140,280,560,1120 mmol/L)of NH_(4)NO_(3) solution for 0(CK),3,6,9,and 12 h,respectively,the changes in seed germination rate,germination index,mean germination time,and seedling vigor index were analyzed to reveal the responses of Bothriochloa ischaemum seed germination to exogenous nitrogen priming.[Result]Nitrogen concentration,priming time and their interaction had extremely significant(P<0.01)effects on the seed germination rate,germination index,mean germination time,and seedling vigor index of Bothriochloa ischaemum.The seed germination rate,germination index,and seedling vigor index of Bothriochloa ischaemum showed an upward trend when the concentrations of NH_(4)NO_(3) solution ranged from 0 to 140 mmol/L,and exhibited a downward trend when the concentrations of NH_(4)NO_(3) solution ranged from 280 to 1120 mmol/L,while the change trend of mean germination time was the opposite.[Conclusion]The seed germination ability of Bothriochloa ischaemum was improved with the lower concentrations(≤140 mmol/L)of NH_(4)NO_(3) solution priming,and was inhibited with the higher concentrations(≥280 mmol/L)of NH_(4)NO_(3) solution priming.The optimal treatment for Bothriochloa ischaemum seeds was priming with NH_(4)NO_(3) solution at concentration of 140 mmol/L for 6 h in production practice.
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