一例喹喔啉酰腙荧光探针对锌离子的选择性高灵敏识别及细胞成像应用  

A quinoxaline‑containing Schiff base probe for the selective and sensitive detection of Zn2+and imaging application in living cells

作　　者：时建朝 田雨蔓 吴伟娜 王元 李小侠[3] SHI Jian-Chao;TIAN Yu-Man;WU Wei-Na;WANG Yuan;LI Xiao-Xia(School of Medicine,Henan Polytechnic University,Jiaozuo,Henan 454000,China;Henan Key Laboratory of Coal Green Conversion,College of Chemistry and Chemical Engineering,Henan Polytechnic University,Jiaozuo,Henan 454000,China;Institute of Functional Materials,Jiangxi University of Finance and Economics,Nanchang 330013,China)

机构地区：[1]河南理工大学医学院,焦作454000 [2]河南理工大学化学化工学院,河南省煤炭绿色转化重点实验室,焦作454000 [3]江西财经大学功能材料研究所,南昌330013

出　　处：《无机化学学报》2023年第7期1295-1302,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21907023,21001040,61964008);江西省自然科学基金(No.20212BAB204017);河南省重点研发与推广专项科技攻关项目(No.232102320092);河南省高校基本科研业务费项目(No.NSFRF230402);河南理工大学安全学科“双一流”创建工程项目(No.AQ20230745,AQ20230754)和河南理工大学研究生教改项目(No.2021YJ16)资助。

摘　　要：通过缩合反应制备了一例席夫碱荧光探针2-喹喔啉甲醛缩2-吡啶酰肼(1),使用核磁共振氢谱和碳谱及质谱等手段表征了探针的结构。荧光光谱分析表明,探针1自身无荧光,而Zn^(2+)能够导致其在500 nm处出现强发射峰。该荧光增强能够在常见阳离子中选择性检测Zn^(2+),检测限低至0.16μmol·L^(-1)。通过核磁、质谱和紫外等手段推测了探针1与Zn^(2+)可能的配位模式。通过单晶X射线衍射解析了1-Zn^(2+)配合物的晶体结构,进一步确认了探针的配位行为。1-Zn^(2+)晶体中探针分别采取ONN和NN配位模式螯合2个Zn^(2+),并由桥联CH3O-和Cl-连接形成一维链状结构。此外,该探针还可用于活细胞中Zn^(2+)的检测。A Schiff base probe,namely N′-(quinoxaline-2-ylmethylene)pyridinyl hydrazide(1)was produced by a simple condensation reaction and was fully characterized through 1H NMR,13C NMR,and ESI-MS.Probe 1 was nonemissive while displaying a strong emission at 500 nm in the presence of Zn^(2+).This fluorescence enhancement was highly selective and sensitive toward Zn^(2+)over other common cations,with a detection limit of 0.16μmol·L^(-1).The coordination mode of 1 with Zn^(2+)was proposed by means of ESI-MS,1H NMR,and UV-Vis.The crystal structure of the 1-Zn^(2+)complex was characterized by X-ray single-crystal diffraction,further confirming the coordination behavior of the probe.The results showed that the probe can coordinate two independent Zn^(2+)ions via ONN and NN donor sets,which are connected by bridged CH3O-and Cl-to form a 1D chain.Finally,the probe was used to trace Zn^(2+)in HeLa cells.

关 键 词：荧光探针 喹喔啉 席夫碱 Zn^(2+) 细胞成像 

分 类 号：O614.241[理学—无机化学]