GOx@GA@MIL-101金属有机骨架用于葡萄糖的快速比色识别  

GOx@GA@MIL‑101 metal‑organic framework for rapid colorimetric recognition of glucose

作　　者：张曼 孙旭建 张欢喜 高雪川 ZHANG Man;SUN Xu-Jian;ZHANG Huan-Xi;GAO Xue-Chuan(Inner Mongolia Engineering Research Center for CO2 Capture and Utilization,Key Laboratory of CO2 Resource Utilization at Universities of Inner Mongolia Autonomous Region,College of Chemical Engineering,Inner Mongolia University of Technology,Hohhot 010051,China)

机构地区：[1]内蒙古工业大学化工学院,二氧化碳资源化利用自治区高等学校重点实验室,内蒙古自治区CO_(2)捕集与资源化工程技术研究中心,呼和浩特010051

出　　处：《无机化学学报》2023年第7期1407-1414,共8页Chinese Journal of Inorganic Chemistry

基　　金：内蒙古自治区自然科学基金(No.2019BS02010);内蒙古自治区直属高校基本科研业务费项目(No.JY20220177);内蒙古工业大学博士启动基金(No.BS201909)资助。

摘　　要：金属有机骨架(MOFs)材料具有均匀的孔隙率和大的比表面积,可作为固定化酶的载体。然而,固定化酶由于较长响应时间或酶易泄漏的缺点阻碍了其应用。本研究选取类过氧化物酶MIL-101为载体,戊二醛(GA)为交联剂,通过交联法将葡萄糖氧化酶(GOx)固定在载体上,建立了模拟多酶体系GOx@GA@MIL-101。制备的复合物可进一步高效催化级联反应检测葡萄糖。GOx@GA@MIL-101具有更快的催化变色效果(30 s)。Metal-organic frameworks(MOFs)with uniform porosity and large surface areas can function as carriers of immobilized enzymes.However,drawbacks including long response times or enzyme leakage have hindered their applicability.In this study,we selected a peroxidase-like MIL-101 as a carrier,glutaraldehyde(GA)as a crosslinking agent,and glucose oxidase(GOx)was immobilized on the carrier by cross-linking method,and a simulated multi-enzyme system GOx@GA@MIL-101was established.The as-prepared composite can further efficiently catalyze the cascade reaction to detect glucose.GOx@GA@MIL-101 had a faster catalytic discoloration effect(30 s).

关 键 词：金属有机骨架 交联法 葡萄糖 比色法检测 级联反应 

分 类 号：O614.811[理学—无机化学]