Green synthesis,characterization,acaricidal,larvacidal,and repellent activities of copper nanoparticles of Astragalus sinicus against Hyalomma anatolicum  

作　　者：Hattan S.Gattan Bassam M.Al-Ahmadi Abdullah F.Shater Qais A.H.Majeed Maha S.Alazemi Abdullah D Alanazi 

机构地区：[1]Department of Medical Laboratory Sciences,Faculty of Applied Medical Sciences,King Abdulaziz University,Jeddah,Saudi Arabia Special Infectious Agents Unit,King Fahad Medical Research Center,Jeddah,Saudi Arabia [2]Department of Biology,Faculty of Science,Taibah University,Saudi Arabia [3]Department of Medical Laboratory Technology,Faculty of Applied Medical Sciences,University of Tabuk,Tabuk 71491,Saudi Arabia [4]Department of Science,College of Basic Education,PAAET,Post code 23167,Aridiya,Kuwait [5]Department of Biological Sciences,Faculty of Science and Humanities,Shaqra University,P.O.Box 1040,Ad-Dawadimi 11911,Saudi Arabia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第6期250-257,共8页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To green synthesize and characterize copper nanoparticles(Cu NPs)using Astragalus sinicus,as well as evaluate the acaricidal,larvacidal,and repellent activities of Cu NPs against Hyalomma anatolicum(H.anatolicum),one of the most prevalent ticks infesting cattle in Saudi Arabia.Methods:Cu NPs were green synthesized by adding the Astragalus sinicus extract to a copper sulfate solution.The acaricidal,larvicidal,and repellent activities of Cu NPs against H.anatolicum were assessed via the adult immersion test,the larval packet test,and the vertical movement behavior of tick larvae,respectively.The effects of Cu NPs on acetylcholinesterase as well as oxidative enzyme activities were examined.Results:The green synthesized Cu NPs displayed a spherical form with a size range of 15-75 nm.After exposure of adult H.anatolicum to different concentrations of Cu NPs,the viability rate of adult H.anatolicum and the mean number,weight,and hatchability of eggs were noticeably reduced,in comparison to the control group(P<0.001).In addition,the viability rate of larvae considerably declined(P<0.001)with the LC_(50)and LC_(90)values of 11.30 and 20.34μg/m L,respectively.The maximum repellent activity of Cu NPs was observed at 50,100,and 200μg/m L with complete repellent activity after 60,120,and 180 min of exposure,respectively.Cu NPs,mainly at 1/2 LC_(50)and LC_(50)concentrations,markedly suppressed the acetylcholinesterase activity of the larval stage of H.anatolicum(P<0.001).Moreover,Cu NPs,mainly at LC_(50)dose,significantly elevated malondialdehyde level while declining glutathione-S-transferase level in H.anatolicum larvae(P<0.001).Conclusions:Cu NPs show potent acaricidal,larvicidal,and repellent activities against adults and larvae of H.anatolicum.However,further studies must be performed to clarify the precise mechanisms and the efficacy of Cu NPs in practical use.

关 键 词：INSECTICIDE TICK PESTICIDE Astragalus sinicus NANOMEDICINE Hyalomma anatolicum Acaricidal activity Larvacidal activity 

分 类 号：TQ454[化学工程—农药化工] TB383.1[一般工业技术—材料科学与工程]