Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital  

作　　者：Nor Syaza Syahirah Amat Junaidi Nik Noorul Shakira Mohamed Shakrin Mohammad Fahmi Daman Huri Ahmad Zakwan Kamarudin Mohd Nasir Mohd Desa Wan Mohd Zin Wan Yunus 

机构地区：[1]Faculty of Medicine and Defence Health,Universiti Pertahanan Nasional Malaysia(UPNM),Kem Sungai Besi,57000 Sungai Besi,Kuala Lumpur,Malaysia [2]Centre for Tropicalization(CENTROP),Universiti Pertahanan Nasional Malaysia(UPNM),Kem Sungai Besi,57000 Sungai Besi,Kuala Lumpur,Malaysia [3]Pathology Department,Tuanku Mizan Armed Forces Hospital(TMAFH),53300 Kuala Lumpur,Malaysia [4]Faculty of Medicine and Health Sciences,Universiti Sains Islam Malaysia(USIM),Persiaran Ilmu,Putra Nilai,71800 Nilai,Negeri Sembilan,Malaysia [5]Department of Biomedical Science,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,43400 Serdang,Selangor,Malaysia [6]Faculty of Defence Science and Technology,Universiti Pertahanan Nasional Malaysia(UPNM),Kem Sungai Besi,57000 Sungai Besi,Kuala Lumpur,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2023年第5期220-231,共12页亚太热带医药杂志（英文版）

基　　金：The study was funded by the UPNM Short Term Grant (UPNM/2019/GPJP/SP/1).

摘　　要：Objective:To determine the antibiotic resistance profile(ARP)of Staphylococcus(S.)aureus isolates and molecular typing of the methicillin-resistant S.aureus(MRSA)isolates from Tuanku Mizan Armed Forces Hospital(TMAFH),Kuala Lumpur.Methods:The ARP and presence of the pvl gene were determined for 209 S.aureus isolates from clinical specimens.Of these,123 were methicillin-susceptible S.aureus(MSSA)isolates and 86 were MRSA isolates.All MRSA isolates were characterized using SCCmec typing and spa typing.Descriptive analysis was performed to compare the demographic data with the phenotypic and genotypic variables of the S.aureus isolates.Results:No vancomycin-intermediate and-resistant S.aureus(VISA and VRSA,respectively)were detected among the study isolates.The MSSA isolates showed low resistance rates to all tested antibiotics,were commonly invasive(28/42,66.7%),and mostly harboured pvl(35/42,83.3%).Meanwhile,MRSA isolates showed high resistance to penicillin(86/86,100%),ampicillin(86/86,100%),sulbactam/ampicillin(86/86,100%),cefuroxime(81/86,94.19%),cefoperazone(76/86,88.37%),azithromycin(56/86,65.12%),and erythromycin(54/86,62.79%).The majority of MRSA isolates were of SCCmec type IVh(65/86,75.58%),spa type t032(55/85,63.95%),and grouped into spaCC-t022(66/85,77.65%).The t032 type was found to be associated with resistance traits to azithromycin and erythromycin(P<0.05).We also found several spa types that are typically associated with hospital-,community-,and livestock-associated MRSA co-existing in our MRSA population.Conclusions:This study reflected the consistent absence of VISA and VRSA and corroborated the clonal shifting of MRSA isolates in the Malaysian MRSA isolates.

关 键 词：Staphylococcus aureus Methicillin-resistant Staphylococcus aureus spa typing SCCmec typing Antibiotic resistance 

分 类 号：R446.5[医药卫生—诊断学]