陕西和辽宁部分地区羊源媒介蜱类及其携带病原体调查  

Investigation on Vector Tick and Tick-Borne Pathogens in Sheep in Some Regions of Shaanxi and Liaoning Province

作　　者：付婷婷 白新鸽 汤芳 刘立明 范君文 刘斌 白杰英 FU Ting-ting;BAI Xin-ge;TANG Fang;LIU Li-ming;FAN Jun-wen;LIU Bin;BAI Jie-ying(Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China;Jilin Province Genet-Med Biotechnology Co.,Ltd.,Changchun 130122,China;School of Polymer Science and Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;Institute of Medical Prevention and Control of Public Health Emergencies,Characteristic Medical Center of the Chinese People's Armed Police Force,Beijing 102613,China;College of Animal Science and Technology,Jilin Agricultural Science and Technology University,Changchun 132101,China;Beijing Animal Disease Prevention and Control Center,Beijing 100020,China;College of Future Technology,Peking University,Beijing 100871,China)

机构地区：[1]中国科学院理化技术研究所,北京海淀100190 [2]吉林省金泰美迪生物技术有限公司,吉林长春130122 [3]青岛科技大学高分子科学与工程学院,山东青岛266042 [4]武警特色医学中心研究部突发公共卫生事件医学防治研究所,北京大兴102613 [5]吉林农业科技学院动物科技学院,吉林长春132101 [6]北京市动物疾病预防控制中心,北京朝阳100020 [7]北京大学未来技术学院,北京海淀100871

出　　处：《中国兽医杂志》2023年第6期1-8,共8页Chinese Journal of Veterinary Medicine

基　　金：病原微生物生物安全国家重点实验室开放基金(SKLPBS2140)。

摘　　要：为了对陕西省和辽宁省部分地区羊源蜱类及其携带病原体的流行情况进行分析,本试验采集了288份羊源蜱样本,采用形态学观察鉴定蜱种;采用分子生物学方法扩增巴贝斯虫18S rRNA、伯氏疏螺旋体16S rRNA、斑点热群立克次体ompA和无形体16S rRNA的基因序列,并结合核苷酸序列分析,确定所收集蜱样本中各类病原体的流行情况。结果显示,经鉴定288份羊源蜱样本均为长角血蜱。陕西省144份长角血蜱中携带巴贝斯虫、斑点热群立克次体和无形体,感染率分别为0.69%、21.53%和77.78%,且巴贝斯虫和斑点热群立克次体均与无形体复合感染;其中长角血蜱中携带Babesia microti、Candidatus Rickettsia longicornis、Uncultured Rickettsia sp.QH-122、Rickettsia endosymbiont of Haemaphysalis longicornis、Uncultured Anaplasma sp.ZJ06/2009、Anaplasma marginale、Anaplasma capra共7种病原体。辽宁省144份长角血蜱中携带斑点热群立克次体和无形体,感染率分别为28.47%和93.06%,且斑点热群立克次体均与无形体复合感染;其中长角血蜱中携带Rickettsia raoultii、Rickettsia endosymbiont of Haemaphysalis longicornis、Anaplasma ovis、Anaplasma bovis共4种病原体。结果表明,陕西省和辽宁省部分地区羊源流行蜱种为长角血蜱,其携带多种病原体,且存在复合感染情况,本结果为防控蜱传疾病提供数据支持。In order to analyze the prevalence of ticks and tick-borne pathogens in sheep in some regions of Shaanxi and Liaoning provinces,a total of 288 tick samples from sheep were collected in this study.Morphological observation was used for tick species identification,and molecular biology methods were employed to amplify the gene sequences of Babesia 18S rRNA,Borrelia burgdorferi 16S rRNA,spotted fever group rickettsia ompA and Anaplasma 16S rRNA,and combined with nucleotide sequence analysis to determine the prevalence of various pathogens in the collected tick samples.The results showed that all 288 tick samples were identified as Haemaphysalis longicornis(H.longicornis).Babesia,spotted fever group rickettsia and Anaplasma were found in 144 H.longicornis from Shaanxi province,with the infection rate of 0.69%,21.53%and 77.78%,respectively;Babesia and spotted fever group rickettsia were found to be co-infected with Anaplasma;among the H.longicornis,a total of seven pathogens were detected,including Babesia microti,Candidatus Rickettsia longicornis,Uncultured Rickettsia sp.QH-122,Rickettsia endosymbiont of H.longicornis,Uncultured Anaplasma sp.ZJ06/2009,Anaplasma marginale and Anaplasma capra.Spotted fever group rickettsia and Anaplasma were found in 144 H.longicornis from Liaoning province,with the infection rates of 28.47%and 93.06%,respectively;and Babesia and spotted fever group rickettsia were found to be co-infected with Anaplasma;among the H.longicornis,a total of four pathogens were detected,including Rickettsia raoultii,Rickettsia endosymbiont of H.longicornis,Anaplasma ovis and Anaplasma bovis.The results indicate that the prevalent tick species in the selected regions of Shaanxi province and Liaoning province are H.longicornis,which carrying multiple pathogens and exhibiting co-infection.These findings provide data support for the prevention and control of tick-borne diseases.

关 键 词：陕西 辽宁 羊 蜱 病原体 

分 类 号：S855.9[农业科学—临床兽医学]