蛋白核小球藻-硝化细菌共培养条件优化与氮磷去除效果  

Optimum condition and nitrogen and phosphorus removal effect of Chlorella pyrenoidosa and nitrifying bacteria mixed culture

作　　者：黄山 杨莹莹[1] 仇志峰 宋志文[1] HUANG Shan;YANG Yingying;QIU Zhifeng;SONG Zhiwen(School of Environmental and Municipal Engineering,Qingdao University of Technology,Qingdao 266525,China;Water Diversion Project Operation and Maintenance Center of Shandong Province,Qingdao 266111,China)

机构地区：[1]青岛理工大学环境与市政工程学院,青岛266525 [2]山东省调水工程运行维护中心,青岛266111

出　　处：《青岛理工大学学报》2023年第3期15-25,共11页Journal of Qingdao University of Technology

基　　金：山东省重点研发计划项目(公益性科技攻关类)(2018GSF117022)。

摘　　要：以氮磷去除为目标,探讨蛋白核小球藻与硝化细菌的适宜共培养条件。采用单因素实验研究了pH、光照强度和光照时间对微藻生物量与氮磷去除效果的影响,通过响应面模型确定了藻菌共培养的最佳条件:pH为8.17、光照强度为5693.31 lux、光照时间为20.67 h,验证该条件下藻细胞、氨氮和总磷浓度分别为4.49×10^(7) cells/mL,0.01 mg/L和1.01 mg/L,与模拟值相对误差均小于3%;该条件下藻菌共培养物对氨氮去除率比单独蛋白核小球藻和硝化细菌实验组分别高16.03%和8.12%,总磷去除率分别高27.22%和89.24%。研究结果可为蛋白核小球藻与硝化细菌共培养物在养殖尾水氮磷去除中的实际应用提供依据。The purpose of this study was to remove nitrogen and phosphorus,and to explore the appropriate mixed culture conditions of Chlorella pyrenoidosa and nitrifying bacteria.The effects of pH,light intensity and light time on the removal of algal biomass,ammonia nitrogen and total phosphorus were studied by single factor experiments.The optimal conditions for mixed culture of algae and bacteria were determined by the response surface model:pH was 8.17,light intensity was 5693.31 lux,and light time was 20.67 h.It was verified that the concentrations of algal cells,ammonia nitrogen and total phosphorus were 4.49×10_(7)cells/mL,0.01 mg/L and 1.01 mg/L respectively,and the relative errors with the simulated values were all less than 3%.Under these conditions,the removal rate of ammonia nitrogen by the algal-bacteria mixed culture was respectively 16.03%and 8.12%higher than that of the single Chlorella pyrenoidosa and single nitrifying bacteria experimental groups,and the total phosphorus removal rate was 27.22%and 89.24%higher,respectively.This study provided theoretical basis and reference for the practical application of algal bacteria mixed culture in nitrogen and phosphorus removal in aquaculture water.

关 键 词：氮磷去除 藻菌共培养 响应面优化 蛋白核小球藻 硝化细菌 

分 类 号：X703[环境科学与工程—环境工程]