hsa-miR-877-3p通过靶向MCM10调控卵巢癌细胞迁移和活性氧产生  

Hsa-miR-877-3p regulates cell migration and reactive oxygen species production of ovarian cancer by targeting MCM10

作　　者：彭琴 崔小波 孙冰纯 罗辉 莫坚 吴斌华 PENG Qin;CUI Xiao-bo;SUN Bing-chun;LUO Hui;MO Jian;WU Bin-hua(Department of Anesthesiology,Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China;Department of Obstetrics andGynecology,Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China;Guangdong ProvincialLaboratory of Southern Marine Science and Engineering,Zhanjiang 524000,China)

机构地区：[1]广东医科大学附属医院麻醉科,广东湛江524001 [2]广东医科大学附属医院妇产科,广东湛江524001 [3]南方海洋科学与工程广东省实验室(湛江),广东湛江524000

出　　处：《广东医科大学学报》2023年第3期259-265,共7页Journal of Guangdong Medical University

基　　金：湛江市科技研究计划项目(2020B01027、2021B01031);南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2019-007);广东医科大学大学生创新实验项目(ZYZF007)。

摘　　要：目的探讨has-miR-877-3p调控卵巢癌细胞迁移和活性氧(ROS)产生的作用机制。方法运用生物信息学分析hsa-miR-877-3p在卵巢癌组织中表达,RT-qPCR检测卵巢癌细胞株中hsa-miR-877-3p表达。构建hsa-miR-877-3p过表达及抑制SKOV3细胞,RNA干扰技术敲低SKOV3细胞中MCM10基因,划痕实验及流式细胞术分别检测细胞迁移、ROS水平。结果hsa-miR-877-3p在卵巢癌中表达低于正常组织(P<0.01),其在卵巢癌细胞株SKOV3、A2780、A1847和8910中表达也降低(P<0.01)。hsa-miR-877-3p过表达抑制SKOV3细胞迁移、增加ROS水平(P<0.01),而抑制hsa-miR-877-3p表达增加SKOV3细胞迁移(P<0.01)。MCM10基因沉默抑制SKOV3细胞迁移、促进ROS表达(P<0.01)。结论hsa-miR-877-3p可以通过调控MCM10基因抑制SKOV3细胞迁移并提高其活性氧水平。Objective To investigate the regulatory mechanism of has-miR-877-3p on cell migration and reactive oxygen species(ROS)of ovarian cancer.Methods Expression of hsa-miR-877-3p in ovarian cancer tissue and cell lines was analyzed by bioinformatics and RT-qPCR,respectively.The hsa-miR-877-3p overexpressed and downexpressed SKOV3 cells were constructed,and MCM10 gene knockdown in SKOV3 cells was performed by RNA interference.The migration and ROS level of SKOV3 cells were dtected by scratch test and flow cytometry,respectively.Results Expression of hsa-miR-877-3p was decreased in ovarian cancer tissue and cell lines including SKOV3,A2780,A1847,and 8910(P<0.01).Overexpressed hsamiR-877-3p inhibited migration and enhanced ROS level of SKOV3 cells,while downexpressed hsa-miR-877-3p increased cell migration(P<0.01).MCM10 gene silencing suppressed migration and reinforced ROS production of SKOV3 cells(P<0.01).Conclusion Hsa-miR-877-3p can decrease migration and increase ROS content of SKOV3 cells by regulating MCM10 gene.

关 键 词：卵巢癌 hsa-miR-877-3p MCM10 活性氧 

分 类 号：Q291[生物学—细胞生物学] Q74