蜜蜂残翅病毒基因组全长克隆及其克隆不稳定性  

The full-length genome cloning and instability of deformed wing virus

作　　者：李润琳 金朗 黄伟峰 LI Runlin;JIN Lang;HUANG Weifeng(College of Animal Science(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2023年第4期519-526,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金项目(2018J01597).

摘　　要：利用长片段PCR技术,以单一病蜂的cDNA为模板,扩增出完整蜜蜂残翅病毒基因组并构建感染性克隆。结果表明,该基因组在某些大肠杆菌内难以稳定克隆,说明蜜蜂残翅病毒分离株具有克隆不稳定性,克隆不稳定性可能仅存在于部分蜜蜂残翅病毒分离株和亚型。国内的蜜蜂残翅病毒分离株在亲缘关系上非常接近,国外的蜜蜂残翅病毒分离株也可能存在克隆不稳定性。The full genome of the deformed wing virus(DWV)was amplified from one diseased bee c DNA sample by long fragment PCR method,and constructed infectious clone.The result shows that it is difficult to obtain a plasmid with a full-length DWV-FY genome in some Escherichia coli,the DWV isolate had instability,the instability may exist in some DWV isolates and subtypes.DWV isolates found in China tended to be grouped together in phylogenetic analyses,suggesting similar instability may exist in other DWV isolates from abroad.

关 键 词：残翅病毒 克隆不稳定性 病毒基因组全长克隆 

分 类 号：S895.2[农业科学—特种经济动物饲养] S852.65[农业科学—畜牧兽医]