单细胞转录组测序技术及其在秀丽隐杆线虫中的应用  

Development of Single Cell Transcriptome Sequencing Technology and Its Application in Caenorhabditis elegans

作　　者：赵金玲 安磊 任晓亮 ZHAO Jin-ling;AN Lei;REN Xiao-liang(Laboratory of Marine Organism Taxonomy and Phylogeny,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071;Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology,Qingdao 266237;University of the Chinese Academy of Sciences,Beijing 100049)

机构地区：[1]中国科学院海洋研究所海洋生物分类与系统演化实验室,青岛266071 [2]青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室,青岛266237 [3]中国科学院大学,北京100049

出　　处：《生物技术通报》2023年第6期158-170,共13页Biotechnology Bulletin

基　　金：国家自然科学基金青年科学基金项目(41906081);青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室人才支持计划项目(YJ2019NO02)。

摘　　要：单细胞转录组测序技术使人们摆脱了细胞异质性问题的干扰,从而在单个细胞水平实现对基因表达及转录调控机制的探索,以及对不同细胞亚群和特殊细胞类型的识别,对系统发育领域的研究具有重要意义。传统模式生物秀丽隐杆线虫(Caenorhabditis elegans)由于体细胞数目固定、细胞分化路径清晰等特点,近年来成为了单细胞转录组学研究的重要模型。本文对目前单细胞转录组测序技术的发展现状进行综述,总结其在秀丽隐杆线虫的细胞谱系分析、单细胞轨迹推断以及神经元细胞图谱等研究工作中的应用情况,并对未来的研究方向作进一步展望。Single cell transcriptome sequencing technology enables people to get rid of the interference of cell heterogeneity,thus realizing the exploration of gene expression and transcriptional regulation mechanism at single cell level,as well as the recognition of different cell subpopulations and special cell types,which is of great significance to the research in the field of phylogeny.The traditional model organism Caenorhabditis elegans has become an important model for single-cell transcriptomic research in recent years because of its fixed number of somatic cells and clear trajectory of cell differentiation.This paper summarizes the development of single-cell transcriptome sequencing technology and its application in the research of cell lineage analysis,single-cell trajectory inference and neuronal cell Atlas of C.elegans,and further prospects the future research direction.

关 键 词：单细胞转录组测序 秀丽隐杆线虫 细胞谱系 

分 类 号：Q23[生物学—细胞生物学]