基于“治未病”理论运用“调肠消瘤方”预防小鼠大肠腺瘤性息肉发生的基础研究  

Basic Research on The Relapse Prevention of Adenomatous Colorectal Polyps in mice with“TiaochangXiaoliu Prescription”Based on The Theory of“Preventive Treatment of Disease”

作　　者：陈克芳[1] 薛小金 Chen Kefang;Xue Xiaojin(Department of Traditional Chinese Medicine,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University Guangzhou 510120)

机构地区：[1]中山大学孙逸仙纪念医院,广东广州510120

出　　处：《科技与健康》2023年第3期8-12,共5页Technology and Health

摘　　要：探究调肠消瘤方对小鼠大肠腺瘤性息肉的发生和相关免疫指标的影响。选择AOM联合DSS的方法建立C57BL/6小鼠大肠腺瘤模型,将健康的70只雄性C57BL/6小鼠,分成空白组(A)、模型组(B)、调肠消瘤方低剂量组(C)、中剂量组(D)、高剂量组(E)和塞来昔布组(F)。治疗组在腹腔注射AOM造模的同时给予中药调肠消瘤方及塞来昔布进行干预,药物浓度为3.5mL/kg、7mL/kg、14mL/kg/d,塞来昔布为20mg/kg/d。采用ELISA法检测小鼠血清中INF-γ、IL-2、TNF-α等因子的含量。研究发现,调肠消瘤方能增加腺瘤性大肠息肉小鼠INF-γ、IL-2、TNF-a含量,提高机体的免疫功能,这可能是调肠消瘤方对大肠腺瘤性息肉术后复发与癌变产生作用的有效机制之一。To investigate the effects of TiaochangXiaoliu prescription on mice adenomatous colonic polyp and related immune function.AOM combined with DSS method was used to establish C57BL/6 mouse adenomatous colorectal polyps model.Seventy healthy male C57BL/6 mouse were divided into blank group(A)、model group(B)、TiaochangXiaoliu prescription low(C)、medium(D)、high-dose group(E)and Celecoxib group(F).The treatment group was received intervention with TiaochangXiaoliu prescription and Celecoxib while injecting AOM intraperitoneally to create a model.The drug concentrations were 3.5ml/kg、7ml/kg、14ml/kg/d and 20mg/kg/d for Celecoxib.Using the method of ELISA to detect the contents of INF-γ、IL-2、TNF-a in mouse serum.TiaochangXiaoliudecoction can increase the contents of INF-γ,IL-2,TNF-A in adenomatous large intestine polyp mice,and enhance the immune function of the body,which may be one of the mechanisms of preventing and treating the recurrence and canceration of adenomatous large intestine polyp.

关 键 词：调肠消瘤方 C57BL/6小鼠 AOM/DSS 大肠腺瘤 免疫功能 

分 类 号：R318.08[医药卫生—生物医学工程]