石菖蒲中α-细辛脑含量测定方法建立及其在开心散中量值传递研究  

Establishment of Content Determination Method ofα-asarum in Acori Tatarinowii Rhizoma and Study on Quality Value Transmitting of Kaixinsan

作　　者：高文雅 冯敏 高畅 赵海誉[1] 周严严[1] 王宏洁[1] 张丽丽 高彦青 肖艳红 程翠娜 魏晓露[1] 边宝林[1] 司南[1] GAO Wenya;FENG Min;GAO Chang;ZHAO Haiyu;ZHOU Yanyan;WANG Hongjie;ZHANG Lili;GAO Yanqing;XIAO Yanhong;CHENG Cuina;WEI Xiaolu;BIAN Baolin;SI Nan(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Sunflower Pharmaceutical Group Co.,Ltd.,Tangshan 064400,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]葵花药业集团(唐山)生物制药有限公司,河北唐山064400

出　　处：《中国中医药信息杂志》2023年第7期132-136,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家中医药管理局中医药防治新型冠状病毒感染的肺炎应急研究专项(2020ZYLCYJ02-9);国家中医药管理局青年岐黄学者支持项目(2021年);中国中医科学院科技创新工程(CI2021A04405、CI2021A04502);中国中医科学院中药研究所横向课题(H2020005-3);杭州市钱江特聘专家项目(2020年)。

摘　　要：目的建立石菖蒲药材、饮片及开心散样品中α-细辛脑的含量测定方法,并考察α-细辛脑在药材→饮片→样品间的量值传递关系。方法采用YMC Hydrosphere C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水(44∶56)为流动相,等度洗脱,流速1.0 mL/min,柱温30℃。结果α-细辛脑在0.197~3.936μg范围内线性关系良好(Y=2×10^(7)X-2707.9,R^(2)=1)。α-细辛脑从石菖蒲药材到饮片的转移率为109.01%,由饮片配制成开心散样品后转移率为104.45%。结论本研究建立的方法适用范围广、简便、重复性好,可用于石菖蒲药材、饮片及开心散样品中α-细辛脑的含量测定,进一步完善石菖蒲药材、饮片及开心散的质量控制标准。Objective To establish a method for the determination ofα-asarum in samples of Acori Tatarinowii Rhizoma and Kaixinsan;To investigate the quality value transmitting ofα-asarum in medicinal materials→decoction pieces→Kaixinsan samples.Methods A YMC Hydrosphere C18 column(4.6 mm×250 mm,5μm)was used,with acetonitrile and water(44∶56)as the mobile phases.The flow rate was 1.0 mL/min,and the column temperature was 30℃.Results The linear relationship ofα-asarum was good in the range of 0.197–3.936μg(Y=2×10^(7)X-2707.9,R^(2)=1).The quality value transmitting ofα-asarum from medicinal materials to decoction pieces after mixed batch was 109.01%,and the quality value transmitting from decoction pieces to Kaixinsan samples was 104.45%.Conclusion The method is widely applicable,simple and repeatable,and can be used for the determination ofα-asarum in the medicinal materials and decoction pieces of Acori Tatarinowii Rhizoma and Kaixinsan samples,which further improves the quality control standard of medicinal materials,decoction pieces of Acori Tatarinowii Rhizoma and Kaixinsan.

关 键 词：石菖蒲 Α-细辛脑 开心散 高效液相色谱法 含量测定 量值传递 

分 类 号：R284.1[医药卫生—中药学]