桉叶油的提取工艺优化及对金黄色葡萄球菌的作用  

Optimization of the Extraction Process of Eucalyptus Oil and its Effect on Staphylococcus Aureus

作　　者：刘瑞秀 覃英克 齐维金 龙利 王鲁 LIU Rui-xiu;QIN Ying-ke;QI Wei-jin;LONG Li;WANG Lu(College of Pharmacy,Guizhou University,Guiyang 550025,China;Engineering Research Center of the Ministry of Education for the Development and Utilization of Southwestern Special Medicinal Biological Resources,Guizhou University,Guiyang 550025,China;Bureau of Agriculture and Rural Affairs,Libo County,Guizhou Province,Libo 558400,China)

机构地区：[1]贵州大学药学院,贵州贵阳550025 [2]贵州大学西南特色药用生物资源开发利用教育部工程研究中心,贵州贵阳550025 [3]贵州省荔波县农业农村局,贵州荔波558400

出　　处：《化学试剂》2023年第7期87-93,共7页Chemical Reagents

基　　金：国家自然科学基金项目(32273051)。

摘　　要：为了评价桉叶油的抑菌作用及最佳提取工艺。通过检测最低抑菌浓度(MIC)考察桉叶油抗菌谱,采用酶标仪测定其对金黄色葡萄球菌(S.aureus)的抑菌曲线,通过检测疏水性、肽聚糖、碱性磷酸酶和β-半乳糖苷酶的含量变化,评价桉叶油对S.aureus细胞壁膜的影响,通过扫描电镜验证(SEM);热回流提取且采用响应面法以油重为评判标准优化桉叶油的工艺条件。结果表明,桉叶油通过改变细胞表面的疏水性,破坏S.aureus细胞壁膜结构的完整性,导致胞内容物外流,引起细胞集聚而抑制S.aureus的生长;优化的桉叶油提取工艺稳定可行。这将为具有良好抗S.aureus活性的桉叶油研发提供理论数据。In order to evaluate the antibacterial effect of eucalyptus oil and the optimal extraction process,the antibacterial spectrum of eucalyptus oil was investigated by detecting the minimum inhibitory concentration(MIC),and its inhibition curve against Staphylococcus aureus(S.aureus)was determined by enzyme standardization,and the effect of eucalyptus oil on the cell wall membrane of S.aureus was evaluated by detecting the changes in the content of hydrophobicity,peptidoglycan,alkaline phosphatase andβ-galactosidase,verified by scanning electron microscopy(SEM).The process conditions of eucalyptus oil were optimized by hot reflux extraction and oil weight as the criterion.The results showed that eucalyptus oil could alter the hydrophobicity of the cell surface and disrupt the membrane structure of S.aureus cell wall.The optimized eucalyptus oil extraction process is stable and feasible.This will provide theoretical data for the development of eucalyptus oil with good anti-S.aureus activity.

关 键 词：桉叶油 金黄色葡萄球菌 细胞壁 细胞膜 提取工艺 

分 类 号：R915[医药卫生—微生物与生化药学]