苦蘵化学成分及抗炎活性研究  

Chemical Constituents of Physalis Angulata L.and Their Anti-inflammatory Activities

作　　者：杨丽婷[1] 李博术 张寒翠 李琼娣 贾敬皓 邱莉[1] 谢集照[1] YANG Li-ting;LI Bo-shu;ZHANG Han-cui;LI Qiong-di;JIA Jing-hao;QIU Li;XIE Ji-zhao(School of Pharmacy,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学药学院,广西南宁530021

出　　处：《化学试剂》2023年第7期94-99,共6页Chemical Reagents

摘　　要：研究苦蘵的化学成分及抗炎活性。采用硅胶、Sephadex LH-20和ODS等柱色谱分离技术对苦蘵乙酸乙酯部位进行分离纯化,通过波谱学技术鉴定化合物结构,并采用LPS诱导的RAW264.7释放NO的细胞模型,评价化合物的体外抗炎活性。从苦蘵中分得13个化合物:5,4′-二羟基-3,3′,7-三甲氧基-黄酮(1)、3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene(2)、(22R)-5β,6β∶14α,17∶14β,26-triepoxy-2α-ethoxy-13,20,22-trihydroxy-1,15-dioxo-16α,24-cyclo-13,14-secoergosta-18,27-dioic acid 18→20,27→22-dilactone(3)、alkesterol B(4)、withaminilide F(5)、酸浆苦素B(6)、酸浆苦素D(7)、酸浆苦素U(8)、withaphysalin R(9)、rel-(18R,22R)-5β,6β∶18β,20-diepoxy-3β,18α-dimethoxy-4β-hydroxy-1-oxowith-24-enolide(10)、withaphysalin P(11)、β-谷甾醇(12)、棕榈酸(13)。化合物1和2首次从酸浆属中分离得到,化合物3~5、10、11首次从该植物中得到。体外抗炎结果表明,化合物5、6、10、11抗炎效果显著。The chemical constituents from Physalis angulata L.and their anti-inflammatory activities were studied.The chemical constituents were isolated and purified by silica gel,Sephadex LH-20 and ODS chromatography.The structures of isolated compounds were elucidated by spectroscopic analyses.Meanwhile the anti-inflammatory activities of the chemical components against NO production from LPS stimulated RAW264.7 cells were evaluated in vitro.The structures were identified as:5,4′-dihydroxy-3,3′,7-trimethoxy-flavone(1),3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene(2),(22 R)-5β,6β∶14α,17∶14β,26-triepoxy-2α-ethoxy-13,20,22-trihydroxy-1,15-dioxo-16α,24-cyclo-13,14-secoergosta-18,27-dioic acid 18→20,27→22-dilactone(3),alkesterol B(4),withaminilide F(5),physalin B(6),physalin D(7),physalin U(8),withaphysalin R(9),rel-(18 R,22 R)-5β,6β∶18β,20-diepoxy-3β,18α-dime-thoxy-4β-hydroxy-1-oxowith-24-enolide(10),withaphysalin P(11),β-sitosterol(12),and palmitic acid(13).Compounds 1 and 2 were isolated form this genus for the first time,while compounds 3~5,10,and 11 were obtained from this plant for the first time.Compounds 5,6,10,and 11 show significant anti-inflammatory activities.

关 键 词：苦蘵 化学成分 酸浆苦素类 醉茄内酯类 抗炎活性 

分 类 号：R284[医药卫生—中药学]