外源2,4-表油菜素内酯对干旱胁迫下桑树根系生长的影响  

Effects of Exogenous 2,4-Epibrassinolide on Root Growth of Mulberry Under Drought Stress

作　　者：董亚茹[1] 孙景诗[1] 王照红[1] 耿兵 赵东晓[1] 李云芝[1] 聂玉霞[1] Dong Yaru;Sun Jingshi;Wang Zhaohong;Geng Bing;Zhao Dongxiao;Li Yunzhi;Nie Yuxia(Shandong Institute of Sericulture,Yantai Shandong 264002,China)

机构地区：[1]山东省蚕业研究所,山东烟台264002

出　　处：《蚕业科学》2023年第2期97-104,共8页ACTA SERICOLOGICA SINICA

基　　金：烟台市科技创新发展计划项目(2021XDHZ057);山东省现代农业产业技术体系蚕桑产业创新团队项目(SDAIT-18-03);财政部和农业农村部国家现代农业产业技术体系项目;山东省农业科学院揭榜科技难题项目(SHJB2022-53)。

摘　　要：为探明外源2,4-表油菜素内酯(2,4-epibrassinolide,EBR)对桑树耐旱性的影响,以杂交桑为试验材料,研究外施不同浓度EBR对15%PEG6000胁迫下桑树幼苗根系生理特性的影响。结果表明:外施EBR能显著缓解干旱胁迫对桑苗根系生长的抑制,其鲜质量、干质量、总根长、表面积、根体积和平均直径比干旱胁迫下提高。与干旱胁迫处理相比,添加0.1μmol/L EBR使桑树幼苗根系产生O_(2)^(·-)活力、H_(2)O_(2)含量、产生·OH能力、丙二醛含量、电导率显著降低,同时使SOD活性、POD活性、CAT活性、脯氨酸含量、可溶性糖含量、可溶性蛋白含量显著增加。结果显示,0.1μmol/L EBR可以缓解15%PEG6000胁迫对桑树幼苗根系的伤害,增强其抗旱性。To illustrate the effect of exogenous 2,4-epibrassinolide(EBR)on the drought tolerance of mulberry,in this study,the effect of different concentrations of EBR on physiological characteristics of mulberry seedling roots under 15% PEG6000 stress were investigated by using hybrid mulberry as the experimental material.Results showed that exogenous 2,4-epibrassinolide could significantly alleviate the inhibition of drought stress on root growth of mulberry seedlings,the fresh weight,dry weight,total root length,surface area,root volume and average diameter of which all increased greatly compared with those under drought stress.With the addition of 0.1μmol/L 2,4-epibrassinolide,the O_(2)^(·-) activity,H_(2)O_(2) content,·OH production capacity,malondialdehyde content and electroconductibility of mulberry seedlings were significantly reduced while the activities of SOD,POD,CAT,and the contents of proline,soluble sugar and soluble protein increased significantly.The results indicated that 0.1μmol/L 2,4-episbrassinolide can enhance the drought resistance by alleviating the damage to root system of mulberry seedlings under 15%PEG6000 drought stress.

关 键 词：桑树 2 4-表油菜素内酯 干旱胁迫 根系 

分 类 号：S888.2[农业科学—特种经济动物饲养]