缺氮与恢复供氮后水稻铵转运蛋白基因的表达特征分析  

Analysis of the Expression Characteristics of Rice Ammonium Transporter Gene After the Treatment of Nitrogen Deficiency and the Recovery of Nitrogen Supply

作　　者：胡太蛟[1] 张珊珊 林艳[1] 宋亚娜[1] HU Tai-jiao;ZHANG Shan-shan;LIN Yan;SONG Ya-na(Institute of Biotechnology,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agricultural Genetic Engineering,Fuzhou,Fujian 350003,China;Crop College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350000,China)

机构地区：[1]福建省农业科学院生物技术研究所/福建省农业遗传工程重点实验室,福建福州350003 [2]福建农林大学作物学院,福建福州350000

出　　处：《福建农业科技》2023年第4期12-21,共10页Fujian Agricultural Science and Technology

基　　金：福建省科技计划公益类项目(2019R1027-2);福建省人民政府、中国农业科学院“5511”协同创新工程(XTCXGC2021002)。

摘　　要：为了解水稻吸收和利用铵态氮的分子机制,利用实时荧光定量PCR检测了水稻全部预测的12条铵转运蛋白基因在缺氮及恢复供氮处理后的表达情况。结果表明:全部铵转运蛋白基因在水稻根、茎和叶中都有表达,AMT1.1在叶中表达水平最高,其表达受到氮缺乏和氮源类型的影响。缺氮处理后,水稻根部的表达全部大幅下调,茎中多数下调,少数上调或波动,叶中大部分先上调再回落;恢复供氮后,水稻根部多数大幅上调,茎和叶中则有所分化,部分上调或先上调再回落。这一现象与水稻氮代谢途径的整体铵转运途径有关,铵的吸收是由根到茎再到叶转运,铵代谢消耗则反向转运。s:In order to understand the molecular mechanism of absorption and utilization of ammonium nitrogen in rice,the real-time fluorescence quantitative PCR was used to detect the expression of all the 12 predicted ammonium transporter genes in rice after the treatment of nitrogen deficiency and nitrogen restoration.The results showed that all the ammonium transporter genes were expressed in the roots,stems and leaves of rice.AMT1.1 had the highest expression level in leaves,and its expression was affected by nitrogen deficiency and the types of nitrogen source.After the treatment of nitrogen deficiency,the expression in rice roots was all significantly down-regulated,the expression in most of the stems were down-regulated,a few were up-regulated or fluctuated,and the expression in most of the leaves were first up-regulated and then down.After restoring the nitrogen supply,the expression in most of the rice roots were significantly up-regulated,while the expression in stems and leaves were differentiated,partially up-regulated or first up-regulated and then down-regulated.This phenomenon was related to the overall ammonium transport pathway of rice nitrogen metabolism pathway.The absorption of ammonium was transported from roots to stems and then to leaves,and the metabolic exhaustion of ammonium was reversed.

关 键 词：表达 实时荧光定量PCR 水稻 AMT基因 氮 

分 类 号：S511[农业科学—作物学]