球等鞭金藻MYB基因家族成员全基因组鉴定和特性分析  

Genome-wide Identification and Characteristic Analysis of MYB Gene Family Members in Isochrysis galbana

作　　者：韩玉莹 陈璐瑶 陈由强 陈多[1,2,3] 陈建楠 HAN Yu-ying;CHEN Lu-yao;CHEN You-qiang;CHEN Duo;CHEN Jian-nan(Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products,Fuzhou,Fujian 350117,China;Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization,Fuzhou,Fujian 350117,China;College of Life Science,Fujian Normal University,Fuzhou,Fujian 350117,China)

机构地区：[1]海洋生物医药与制品产业化开发技术公共服务平台,福建福州350117 [2]福建省特色海洋生物资源可持续利用重点实验室,福建福州350117 [3]福建师范大学生命科学学院,福建福州350117

出　　处：《福建农业科技》2023年第4期22-35,共14页Fujian Agricultural Science and Technology

基　　金：国家现代农业产业技术体系项目(CARA-170501)。

摘　　要：为了深入理解球等鞭金藻MYB基因家族在基因调控网络中的作用机制,使用MYB_DNA-binding保守结构域在球等鞭金藻蛋白序列中筛选出60个MYB基因家族成员,并对这些基因家族成员的蛋白(一、二、三级)结构预测和分析、亚细胞结构定位、基因结构及保守结构域等方面进行了分析。结果表明:这些MYB基因家族成员可分为3个cluster,其中R2-MYB和R3-MYB主要集中在cluster 3中,R2R3-MYB则含有大部分家族成员,表明这些基因在球等鞭金藻中起着重要的生物学功能。不同分支的亲缘关系较远,同一分支的关系较近,这表明球等鞭金藻MYB基因家族在演化过程中形成了一些适应性的变化。通过基因组内共线性分析,研究发现球等鞭金藻全基因组内有177对片段复制基因和46对串联复制基因,其中MYB基因家族有2对复制基因,包括串联重复基因对IZ004267和IZ007345以及片段重复基因对IZ006462和IZ006102。结合染色体定位分析,IZ006462和IZ006102位于9号染色体,这也支持上述片段重复的判断。这些重复基因对的K a/K s值小于1,表明这些基因在扩张过程中受到了基因纯化选择,功能相当保守。In order to further understand the mechanism of the MYB gene family in the gene regulatory network,the MYB_DNA-binding conserved domain was used to screen 60 MYB gene family members from the protein sequences of Isochrysis galbana.Then,the protein(primary,secondary and tertiary)structure prediction and analysis,subcellular structure localization,gene structure,and conserved domains of these gene family members were analyzed.The results showed that:these MYB gene family members could be divided into three clusters,among which R2-MYB and R3-MYB were mainly concentrated in cluster 3,and R2R3-MYB contained most of the family members,indicating that these genes played an important biological function in Isochrysis galbana.The distant relationship between different branches and the close relationship within the same branch indicated that the MYB gene family in Isochrysis galbana had undergone some adaptive changes during the evolution process.Through the analysis of collinearity in the genome,the study found that there were 177 pairs of segmental duplication genes and 46 pairs of tandem duplication genes in the whole genome of Isochrysis galbana,among which there were 2 pairs of duplication genes in the MYB gene family,including the tandem duplicated gene pair IZ004267 and IZ007345,and the segmental duplicated gene pair IZ006462 and IZ006102.By combining with the analysis of chromosomal localization,IZ006462 and IZ006102 were located on chromosome 9,which also supported the above judgment of segmental duplication.The Ka/Ks values of these duplicated gene pairs were less than 1,indicating that these genes were subjected to gene purification selection during expansion and their functions were quite conserved.

关 键 词：球等鞭金藻 MYB基因家族 全基因组 光质 表达分析 

分 类 号：Q943.2[生物学—植物学]