基于加权基因共表达网络分析鉴定调控油菜种子次生休眠候选基因  

Identification of candidate genes regulating rapeseed secondary dormancy by weighted gene co⁃expression network analysis

作　　者：谷玉娟 刘磊 赵祥祥[2] 刘福霞[2] 路芳芳 梁大安 郝天琪 高倩 吴德鹏 高慧 GU Yu-juan;LIU Lei;ZHAO Xiang-xiang;LIU Fu-xia;LU Fang-fang;LIANG Da-an;HAO Tian-qi;GAO Qian;WU De-peng;GAO Hui(Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;Jiangsu Collaborative Inno-vation Center of Regional Modern Agriculture&Environmental Protection,Huaiyin Normal University,Huai'an 223300,China)

机构地区：[1]河北科技师范学院,河北秦皇岛066004 [2]淮阴师范学院江苏省区域现代农业与环境保护协同创新中心,江苏淮安223300

出　　处：《中国油料作物学报》2023年第3期524-532,共9页Chinese Journal of Oil Crop Sciences

基　　金：江苏省农业自主创新项目(CX(21)3118);江苏省区域现代化农业与环境保护协同创新中心团队集体攻关项目(HSXT30123);国家自然科学基金(31701514)。

摘　　要：油菜种子的次生休眠容易导致油菜自生苗发生,影响制种品质和安全。本研究以课题组自主培育的Huaiyou-WSD-H2(次生休眠弱)和Huaiyou-SSD-V1(次生休眠强)品系为材料,利用聚乙二醇(polyethylene glycol,PEG)模拟干旱,诱导种子次生休眠,对次生休眠诱导前、后的油菜种子进行高通量转录组测序。利用加权基因共表达网络分析(weighted gene co-expression network analysis,WGCNA)将高通量转录组测序中17706个高表达基因(FPKM>5)划分为17个共表达模块。结合GO/KEGG富集分析和差异表达分析,在与次生休眠极显著正相关的yellow模块中筛选到3个与色氨酸代谢相关的基因(BnaC08g25400D、BnaC09g31260D和BnaC09g49740D)在诱导后的强、弱次生休眠材料间存在差异表达,利用qRT-PCR进行验证,并初步分析了候选基因的调控网络,为解析依赖色氨酸的生长素生物合成途径调控油菜种子次生休眠的遗传基础提供理论依据。Rapeseed(Brassica napus L.)exhibits different degrees of secondary dormancy,which,if strong,can easily lead to the occurrence of volunteer plants in field and affect the quality and safety of seed production.In this study,we used Huaiyou-WSD-H2(weak secondary dormancy)and Huaiyou-SSD-V1(strong secondary dormancy)lines,which are derived from two breeding lines,to induce secondary dormancy using polyethylene glycol(PEG)to simulate drought,and conducted high-throughput transcriptome sequencing.Totally 17706 highly expressed genes(FPKM>5)from the high-throughput transcriptome sequencing were divided into 17 co-expression modules using weighted gene co-expression network analysis(WGCNA).Combined with GO/KEGG enrichment analysis and differ-ential expression analysis,3 genes related to tryptophan metabolism(BnaC08g25400D,BnaC09g31260D and BnaC09g49740D)were screened in the yellow module,which was highly significantly and positively correlated with secondary dormancy,and were differentially expressed between strong and weak secondary dormant materials after induction.Validation by qRT-PCR and preliminary analysis of the regulatory network of candidate genes were per-formed to provide a theoretical basis for resolving the genetic basis of tryptophan-dependent growth hormone biosyn-thesis pathway to regulate secondary seed dormancy in rapeseed.

关 键 词：甘蓝型油菜 次生休眠 加权基因共表达网络分析 转录组 

分 类 号：S565.4[农业科学—作物学]