猴樟根系适应碱胁迫的代谢组学分析  

Metabolomic Analysis of Cinnamomun bodinieri Root Adapted to Alkali Stress

作　　者：韩浩章[1] 张丽华[1] 李素华[1] 赵荣 王芳[1] Han Haozhang;Zhang Lihua;Li Suhua;Zhao Rong;Wang Fang(Suqian University,Suqian 223800,P.R.China)

机构地区：[1]宿迁学院,宿迁223800

出　　处：《东北林业大学学报》2023年第7期24-32,共9页Journal of Northeast Forestry University

基　　金：江苏省自然科学基金项目(BK20201481);宿迁市科技计划项目(L202004、M202001);宿迁学院重点建设学科项目(2021ZDJS04);宿迁学院创新团队项目(2021td05)。

摘　　要：为阐明猴樟适应碱胁迫的代谢机制,培育耐碱品种,扩大推广面积,采用液相色谱-质谱对碱胁迫的猴樟根系差异代谢产物和代谢通路进行研究,分析猴樟适应碱胁迫的代谢机制。结果表明:碱胁迫时,差异代谢物主要包括生物碱类、聚酮类、有机酸类、核酸、苯丙素类、脂类、抗生素和萜类;京都基因与基因组百科全书(KEGG)和差异丰度得分结果显示,当碱胁迫处理6 h时,差异代谢物KEGG通路中大部分显著下调或趋于下调,只有脂肪酸生物合成和丙氨酸、天冬氨酸和谷氨酸代谢通路显著上调,托烷、哌啶和吡啶生物碱的生物合成通路、嘧啶代谢通路、磷酸转移酶系统通路和苯丙烷生物合成通路趋于上调;当碱胁迫处理48 h时,差异代谢物KEGG通路中只有半乳糖代谢通路和黄酮类生物合成通路趋于上调,其他通路均显著下调或趋于下调。因此,猴樟通过提高氨基酸、糖类、有机酸和生物碱的质量分数适应初期碱胁迫,通过抗氧化活性抵抗后期碱胁迫。In order to clarify the metabolic mechanism of Cinnamomun bodinieri in response to alkali stress,cultivate alkali resistant varieties and expand the popularization area.The liquid chromatography-mass spectrometry was used to study the different metabolites and metabolic pathways of C.bodinieri monassana under alkali stress,and to analyze the metabolic differences of C.bodinieri monassana under alkali stress.The results showed that under alkali stress,the differential metabolites mainly included alkaloids,polyketides,organic acids,nucleic acids,phenylpropanoids,lipids,antibiotics and terpenoids.The results of KEGG and DAScore plots showed that when alkali stress was treated for 6 h,most of the KEGG pathways of differential metabolites were significantly down-regulated or tended to be down-regulated,and only fatty acid biosynthesis and alanine,aspartate and glutamate metabolism pathways were significantly up-regulated.The biosynthesis pathway of trotane,piperidine and pyridine alkaloids,pyrimidine metabolism pathway,phosphotransferase system(PTS)pathway and phenylpropane biosynthesis pathway tended to be up-regulated.When alkali stress was treated for 48 h,only galactose metabolism pathway and flavonoid biosynthesis pathway tended to be up-regulated,while other pathways were significantly down-regulated or tended to be down-regulated.Therefore,C.bodinieri can adapt to the initial alkali stress by increasing the content of amino acids,sugars,organic acids and alkaloids,and C.bodinieri is mainly antioxidant stress in the later alkali stress.

关 键 词：猴樟根系 碱胁迫 代谢组学 

分 类 号：S792.23[农业科学—林木遗传育种]