RP-HPLC测定眩晕宁颗粒制剂中3种成分的含量  

RP-HPLC determination of three components in Xuanyunning granules

作　　者：倪恒雨 Ni Hengyu(Huainan Food and Drug Inspection Center,232007)

机构地区：[1]淮南市食品药品检验中心,232007

出　　处：《现代科学仪器》2023年第3期32-36,共5页Modern Scientific Instruments

摘　　要：目的:建立了RP-HPLC法测定眩晕宁颗粒制剂中异绿原酸A等3种成分含量的方法。方法:CapcellPak UG C18色谱柱分离;检测波长:异绿原酸A、23-乙酰泽泻醇B和β-蜕皮甾酮检测波长分别设定348 nm、250 nm和208 nm;进样体积:10µL;柱温:28℃;以乙腈(A)-0.5%磷酸溶液(B)为洗脱溶剂,外标法定量检测。结果:异绿原酸A、β-蜕皮甾酮和23-乙酰泽泻醇B分别在浓度0.0767~7.67、0.0983~9.83和0.0197~1.97μg/mL范围内线性良好;回收率依次为100.05%、98.33%和99.42%;重复性和精密度RSD均在5.0%以内;供试品溶液在24h内稳定。结论:结果证明,此方法具有前处理简单等优点,可以用于眩晕宁颗粒制剂的质量控制。Objective:A RP-HPLC method was established for the determination of 3,5-O-dicaffeoylquinic acid in Xuanyunning granules.Method:Capcell Pak UG C18 chromatographic column for separation;Detection wavelength:The detection wavelength of 3,5-O-dicaffeyl quinic acid,23-acetyl xerol B andβ-ecdysteroid is set at 348 nm,250 nm and 208 nm respectively;Column temperature:28℃;Injection volume:10μL;Acetonitrile is used as mobile phase a,0.5%phosphoric acid solutionis used as mobile phase B,external standard method for quantitative detection.Result:3,5-O-dicaffeyl quinic acid,23-acetyl xerol B andβ-ecdysteroid showed good linear relationships in the concentration range 0.0767~7.67、0.0983~9.83 and 0.0197~1.97μg/mL.Average recoveries were 100.05%,98.33%and 99.42%respectively;RSD(n=6)of precision and repeatability are within 5.0%,The test solution is stable within 24h.Conclusion:The results showed that this method has the advantages of simple pretreatment and can be used for the quality control of Xuanyunning granules.

关 键 词：RP-HPLC 眩晕宁颗粒 异绿原酸A 23-乙酰泽泻醇B β-蜕皮甾酮 

分 类 号：R284.1[医药卫生—中药学]