用于快速分离血浆的阶梯型螺旋微流道  

Stepped spiral microchannels for rapid blood plasma separation

作　　者：张鑫杰 朱行杰[1] 刘尧 顾乔 张宇航 Oseyemi Ayobami Elisha 吕芳蕊 倪中华 Zhang Xinjie;Zhu Hangjie;Liu Yao;Gu Qiao;Zhang Yuhang;Oseyemi Ayobami Elisha;LüFangrui;Ni Zhonghua(College of Mechanical and Electrical Engineering,Hohai University,Changzhou 213022,China;School of Mechanical Engineering,Southeast University,Nanjing 211189,China;Department of Gynecology and Obstetrics,The Third Affiliated Hospital of Soochow University,Changzhou 213003,China;Department of Mechanical Engineering,York University,Toronto,ON M3J1P3,Canada)

机构地区：[1]河海大学机电工程学院,常州213022 [2]东南大学机械工程学院,南京211189 [3]苏州大学附属第三医院妇产科,常州213003 [4]Department of Mechanical Engineering,York University,Toronto,ON M3J1P3,Canada

出　　处：《Journal of Southeast University(English Edition)》2023年第2期176-186,共11页东南大学学报（英文版）

基　　金：The National Natural Science Foundation of China(No.51905150);Fundamental Research Funds for the Central Universities(No.B220202024);Changzhou Science and Technology Bureau Program(No.CE20225046),Changzhou Health Commission Youth Science and Technology Projects(No.QN202115);Jiangsu Planned Projects for Postdoctoral Research Funds(No.2019K033),Postgraduate Research and Practice Innovation Program of Jiangsu Province(No.KYCX23_0670).

摘　　要：为解决传统血液处理方法如离心和过滤等存在处理时间、分离纯度、堵塞性等方面限制的问题,设计一种高通量惯性微流控芯片,该芯片由阶梯型螺旋流道组成,可用于高浓度血浆分离.首先,采用COMSOL Multiphysics软件的层流和粒子追踪模块仿真研究阶梯型螺旋流道中粒子的聚焦特性.然后,通过实验研究了不同流量下聚苯乙烯粒子在流道中的惯性聚焦行为.最后,基于粒子聚焦实验结果,选择了一种优化结构的阶梯型螺旋流道,以用于不同浓度血液中血浆的分离应用.实验发现,在最优流量1.5 mL/min时,血细胞容积为0.9%和2.25%的血样中血细胞移除率分别达到(99.72±0.13)%和(99.44±0.17)%.当血细胞容积高达4.5%和9.0%时,该阶梯型螺旋流道仍然具有(97.02±0.56)%和(92.92±1.53)%的血细胞移除率.实验结果表明,阶梯型螺旋流道可以从高浓度血样中高效分离血浆.Conventional ways of blood processing,such as centrifugation and filtration,are fairly limited by processing time,separation purity,clogging,and other factors.To solve this problem,a high-throughput,inertial microfluidic device composed of a stepped spiral channel is proposed for the separation of plasma from high-concentration blood.First,the particle-focusing characteristics of the stepped spiral channel are studied through the coupling of laminar flow and particle tracking modules in the COMSOL Multiphysics software.Next,polystyrene beads are used to investigate the inertial focusing performances of the stepped spiral channel at different flow rates.Based on the experimental results of particle focusing,an optimized stepped spiral channel is applied in blood plasma separation from samples of different cell concentrations.It can be observed that the reject ratios of the blood cells are(99.72±0.13)%and(99.44±0.17)%with hematocrit(HCT)values of 0.9%and 2.25%,respectively,at an optimal flow rate of 1.5 mL/min.The ratios of blood cells rejected by the stepped spiral channel are(97.02±0.56)%and(92.92±1.53)%at ultrahigh HCT values of 4.5%and 9%,respectively.The experimental findings demonstrate that the stepped spiral channel can efficiently separate plasma from ultrahigh-concentration blood samples.

关 键 词：微流控 惯性聚焦 阶梯型螺旋流道 血浆分离 二次流调控 

分 类 号：TH77[机械工程—仪器科学与技术]