应用非靶向代谢组学技术分析经铵态氮胁迫处理柳树根系的差异代谢物  

Metabolite Differences of Willow Roots under Ammonia Nitrogen Stress Using Non-targeted Metabolomics

作　　者：王红玲[1] 黄瑞芳[1] 施士争[1] Wang Hongling;Huang Ruifang;Shi Shizheng(Jiangsu Academy of Forestry,Nanjing 211153,P.R.China)

机构地区：[1]江苏省林业科学研究院,南京211153

出　　处：《东北林业大学学报》2023年第8期48-52,共5页Journal of Northeast Forestry University

基　　金：国家科技支撑计划项目(2015BAD07B050403)。

摘　　要：为揭示铵态氮对柳树根系的影响机制,以柳树无性系为试验材料,分别用低浓度、高浓度的铵态氮胁迫处理后,采用超高效液相色谱-质谱联用技术对柳树根系进行非靶向代谢组学研究。结果表明:低质量浓度铵态氮(2 mg·L^(-1))胁迫处理时,鉴定出28种差异代谢物;高质量浓度铵态氮(20 mg·L^(-1))胁迫处理时,鉴定出216种差异代谢物,其中多元醇和脂肪酸类小分子代谢物的差异较大。结合京都基因与基因组百科全书(KEGG)数据库进一步对差异代谢物进行通路富集分析,差异代谢物主要参与不饱和脂肪酸的生物合成、植物激素的合成与信号转导、α-亚麻酸的代谢、糖胺聚糖生物合成等途径。结果表明:低质量浓度铵态氮对柳树根系的代谢物影响较小,而高质量浓度铵态氮对柳树根系的代谢物影响较大,柳树根系主要通过调节植物激素、细胞渗透压等方式来应对铵态氮胁迫。In order to investigate the impact mechanisms of ammonia nitrogen on willow roots,willow roots were treated with low and high content of ammonium nitrogen,respectively.The metabolic components were detected using high-performance liquid chromatography coupled with tandem mass spectrometry.The results showed that 28 different metabolites were identified under low concentration(2 mg·L^(-1))of ammonium nitrogen stress.Under high concentration(20 mg·L^(-1))of ammonium nitrogen stress,216 different metabolites were identified,among which polyol and fatty acid related small molecule metabolites were significantly different.Combined with KEGG database,the pathway enrichment analysis of differential metabolites was further carried out.Differential metabolites mainly participated in biosynthesis of unsaturated fatty acids,biosynthesis of plant hormones,plant hormone signal transduction,alpha-linolenic acid metabolism,and glycosaminoglycan biosynthesis.The results showed that low content of ammonium nitrogen had little effect on the metabolites of willow roots,while high content of ammonium nitrogen had greater effects.Willow roots mainly responded to ammonium nitrogen stress by regulating plant hormones and cell osmotic pressure.

关 键 词：铵态氮胁迫 非靶向代谢组学 代谢物 

分 类 号：S718.4[农业科学—林学]