白及正丁醇提取物止血作用的谱效关系研究  

Study on the Spectrum Effect Relationship of Haemostatic Action of n-Butanol Extract of Bletillae Rhizoma

作　　者：刘林莉 麻秀萍 徐剑 张永萍 刘静 钱松 孙毕 LIU Lin-li;MA Xiu-ping;XU Jian;ZHANG Yong-ping;LIU Jing;QIAN Song;SUN Bi(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National Engineering Technology Research Center for Miao Medicine/Guizhou Engineering Technology Research Center for Processing and Preparation of Traditional Chinese Medicine and Ethnic Medicine,Guiyang 550025,China;Guizhou University of Traditional Chinese Medicine Tea with Health Food Development Research Center,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]国家苗药工程技术研究中心/贵州中药炮制与制剂工程技术研究中心,贵州贵阳550025 [3]贵州中医药大学茶+大健康食品开发研究中心,贵州贵阳550025

出　　处：《中药材》2022年第11期2664-2670,共7页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金委员会-贵州省人民政府联合基金项目(U1812403-2-4);贵州省国内一流学科建设项目(GNYL[2017]008);贵州省药物新剂型新工艺科技创新人才团队项目(黔科合平台人才[2017]5655)。

摘　　要：目的:采用HPLC和UPLC-Q-TOF-MS/MS方法分析白及正丁醇提取物,与止血作用相关联,明确白及止血物质基础。方法:建立16批白及正丁醇提取物HPLC指纹图谱,以毛细管法和断尾法考察小鼠凝血和出血时间,评价止血作用;采用灰色关联度分析法、双变量相关分析法及偏最小二乘回归分析法研究谱效关系。结果:白及正丁醇提取物有15个共有峰,其中1、8、9、11、13~15号色谱峰与止血作用的关联度>0.6且Pearson相关系数为正,利用质谱数据结合对照品确定1号为天麻素、8号为gymnosideⅠ、9号为militarine,偏最小二乘回归分析确定1、4、8~10号色谱峰与止血时间呈负相关,且VIP值>1。结论:白及止血活性成分为gymnosideⅠ、militarine,该研究可为提升白及质量标准和进一步药效研究提供参考。Objective:To analyze the n-butanol extract of Bletillae Rhizoma by HPLC and UPLC-Q-TOF-MS/MS methods,to associated with hemostatic effect and clarify the hemostatic material basis of Bletillae Rhizoma.Methods:HPLC fingerprint of 16 batches of Bletillae Rhizoma n-butanol extracts was established,and the blood coagulation and bleeding times of mice were evaluated by capillary method and tail-breaking method.Grey correlation analysis,bivariate correlation analysis and partial least squares regression analysis were used to study the spectrum effect relationship.Results:There were 15 common peaks in the n-butanol extract of Bletillae Rhizoma,among which the correlation degree of peaks 1,8,9,11,13 to 15 with haemostatic action was greater than 0.6 and the Pearson correlation coefficient was positive.Using mass spectrometry data combined with reference substance,peak 1 was gastrodin,peak 8 was gymnoside Ⅰ and peak 9 was militarine.Partial least squares regression further analysis showed that peaks 1,4,8 to 10 were negatively correlated with hemostasis action and VIP value was greater than 1.Conclusion:The hemostatic active components of Bletillae Rhizoma are the chemical constituents represented by gymnoside Ⅰ and militarine.This study provides a scientific basis for improving the quality standard and further pharmacodynamic study of Bletillae Rhizoma.

关 键 词：白及 止血作用 指纹图谱 谱效关系 UPLC-Q-TOF-MS/MS 

分 类 号：R284.1[医药卫生—中药学]