河南省禹州地区白纹伊蚊击倒抗性基因突变检测  

Detection of knockdown resistance gene mutations in Aedes albopictus in Yuzhou,Henan province,China

作　　者：母群征 华栋栋 李文玉 周欣欣 伦辛畅 李贵昌[1] 王君[1] 宋秀平[1] 刘起勇[1] 孟凤霞[1] MU Qun-zheng;HUA Dong-dong;LI Wen-yu;ZHOU Xin-xin;LUN Xin-chang;LI Gui-chang;WANG Jun;SONG Xiu-ping;LIU Qi-yong;MENG Feng-xia(State Key Laboratory of Infectious Disease Prevention and Control,Department of Vector Biology and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,WHO Collaborating Centre for Vector Surveillance and Management,Beijing 102206,China;School of Public Health,Shandong University,Ji’nan,Shandong 250000,China;Ningxia Center for Disease Prevention and Control,Yinchuan,Ningxia 750000,China;Daxing District Center for Disease Control and Prevention of Beijing,Beijing 102600,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所媒介生物控制室,传染病预防控制国家重点实验室,世界卫生组织媒介生物监测与管理合作中心,北京102206 [2]山东大学公共卫生学院,山东济南250000 [3]宁夏回族自治区疾病预防控制中心,宁夏银川750000 [4]北京市大兴区疾病预防控制中心,北京102600

出　　处：《中国媒介生物学及控制杂志》2023年第3期303-307,335,共6页Chinese Journal of Vector Biology and Control

基　　金：国家科技重大专项(2018ZX101010002-002)。

摘　　要：目的了解河南省禹州地区白纹伊蚊击倒抗性基因突变情况,为白纹伊蚊防控提供依据。方法2020和2022年9月中旬在禹州地区利用双层叠帐法捕获蚊虫;依据形态特征鉴定蚊虫种类,提取白纹伊蚊DNA,通过测序法对白纹伊蚊击倒抗性基因进行检测。结果本次调查每年捕获40只共计80只白纹伊蚊。测序结果表明白纹伊蚊3个击倒抗性基因位点均有发现。1016位点存在由密码子GTA编码的缬氨酸(Val,V)突变为GGA编码的甘氨酸(Gly,G);1532位点存在由密码子ATC编码的异亮氨酸(Ile,I)突变为ACC编码的苏氨酸(Thr,T);1534位点突变等位基因型最多,包括由密码子TTC(Phe,F)编码的苯丙氨酸突变为TCC编码的丝氨酸(Ser,S)或TTA/CTC编码的亮氨酸(Leu,L);3个突变位点共包括10种基因型。3个突变位点均是以野生型纯合子为主,野生/突变型杂合子次之,突变型纯合子所占比例最低。共得到11种组合基因型,其中单个位点野生/突变杂合型组合基因型频率最高,为55.00%,3个位点的野生纯合型组合基因型频率为28.75%;双位点野生/突变杂合型基因型频率为8.75%,单个位点突变纯合型基因型频率最低,为7.50%。结论首次在禹州地区开展白纹伊蚊击倒抗性基因突变检测,发现其基因突变率高且突变情况复杂,今后应在该地区开展深入调查研究。Objective To investigate the knockdown resistance gene mutations of Aedes albopictus in Yuzhou,Henan province,China,and to provide a basis for the control of Ae.albopictus.Methods The double-layered mosquito net method was used to capture mosquitoes in Yuzhou in mid-September of 2020 and 2022.Mosquito species was identified based on morphological characteristics.The DNA of Ae.albopictus mosquitoes was extracted for knockdown resistance gene detection by sequencing.Results A total of 80 Ae.albopictus mosquitoes(40 in each year)were captured.The sequencing results showed that three knockdown resistance gene loci were found mutant in Ae.albopictus.The 1016 locus mutated from valine(Val,V)encoded by the codon GTA to glycine(Gly,G)encoded by GGA.The 1532 locus mutated from ATC-encoded isoleucine(Ile,I)to ACC-encoded threonine(Thr,T).Mutations at 1534 were most frequent,from TTC-encoded phenylalanine(Phe,F)to TCC-encoded serine(Ser,S)or Leu(Leu,L)encoded by TTA/CTC.There were 10 genotypes in total for the three mutant sites.For all the three mutant sites,wild-type homozygotes were predominant,followed by wild/mutant heterozygotes and then mutant homozygotes.There were a total of 11 combination genotypes:the frequency of wild/mutant heterozygous combination genotypes at single loci was 55.00%,which was highest;the frequency of wild homozygous combination genotypes at three loci was 28.75%;the frequency of wild/mutant heterozygous genotypes at two loci was 8.75%;and the frequency of mutant homozygous genotypes at single loci was 7.50%,which was lowest.Conclusions It was the first time to carry out knockdown resistance gene mutation detection in Ae.albopictus in Yuzhou,discovering that the mutations were frequent and complex,which requires further research in this area.

关 键 词：白纹伊蚊 击倒抗性基因 禹州市 

分 类 号：S443[农业科学—农业昆虫与害虫防治]