HPLC-MS/MS法同时测定清心滋肾方中27种成分  

Simultaneous determination of twenty-seven constituents in Qinxin Zishen Prescription by HPLC-MS/MS

作　　者：商娟 李雪娇 顾潇 李臻阳 袭晓昀 姚卫峰[2] 邹建东[1] 卢苏[1] 陈赟[1] 许美娟[1] SHANG Juan;LI Xue-jiao;GU Xiao;LI Zhen-yang;XI Xiao-yun;YAO Wei-feng;ZOU Jian-dong;LU Su;CHEN Yun;XU Mei-juan(The Hospital Affiliated to Nanjing University of Chinese Medicine(Jiangsu Provincial Hospital of Traditional Chinese Medicine),Nanjing 210029,China;Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学附属医院(江苏省中医院),江苏南京210029 [2]南京中医药大学,江苏南京210023

出　　处：《中成药》2023年第6期1774-1780,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81574009);江苏省中医药科技发展计划重点项目(ZD202206);江苏省卫计委科研课题(Q201602);“十四五”江苏省重点学科项目—公共卫生与预防医学(035091005007);南京中医药大学自然科学基金面上项目(XZR2020015)。

摘　　要：目的建立HPLC-MS/MS法同时测定清心滋肾方中去甲乌药碱、莲心季胺碱、N-甲基衡州乌药碱、莲心碱、木兰花碱、异莲心碱、甲基莲心碱、斯皮诺素、表小檗碱、黄连碱、去氢钩藤碱、异钩藤碱、钩藤碱、巴马汀、小檗碱、缝籽嗪甲醚、梓醇、没食子酸、丹参素、原儿茶酸、原儿茶醛、莫诺苷、马钱苷、紫草酸、迷迭香酸、丹酚酸B、丹酚酸A的含量。方法该药物水煎液的分析采用Agilent ZORBAX SB C 18色谱柱(4.6 mm×100 mm,1.8μm);流动相0.5%甲酸-[甲醇-乙腈(1∶1)],梯度洗脱;体积流量0.5 mL/min;柱温40℃;电喷雾离子源;正负离子扫描;多反应监测模式。结果27种成分在各自范围线性关系良好(r≥0.9956),平均加样回收率90.2%~105.3%,RSD 1.6%~9.1%。结论该方法快速准确,可靠灵敏,可为清心滋肾方质量控制提供参考依据。AIM To establish an HPLC-MS/MS method for the simultaneous content determination of norcoclaurine,lotusine,N-methylcoclaurine,liensinine,magnoflorine,isoliensinine,neferine,spinosin,epiberberine,coptisine,corynoxeine,isorhynchophylline,rhynchophylline,palmatine,berberine,geissoschizine methyl ether,catapol,gallic acid,danshensu,protocatechuic acid,protocatechualdehyde,morroniside,loganin,lithospermic acid,rosmarinic acid,salvianolic acid B and salvianolic acid A in Qinxin Zishen Prescription.METHODS The water decoction of this drug was performed on a 40℃thermostatic Agilent ZORBAX SB C 18 column(4.6 mm×100 mm,1.8μm),with the mobile phase comprising of 0.5%formic acid-[methanol-acetonitrile(1∶1)]flowing at 0.5 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Twenty-seven constituents showed good linear relationships within their own ranges(r≥0.9956),whose average recoveries were 90.2%-105.3%with the RSDs of 1.6%-9.1%.CONCLUSION This rapid,accurate,reliable and sensitive method can provide reference basis for the quality control of Qinxin Zishen Prescription.

关 键 词：清心滋肾方 化学成分 HPLC-MS/MS 

分 类 号：R927.2[医药卫生—药学]