穗花牡荆的种实解剖结构及催芽技术研究  

Research on the seed anatomy and germination technology of Vitex agnus-castus L.

作　　者：马琳[1,2] 王啸博 刘秀丽 Ma Lin;Wang Xiao-Bo;Liu Xiu-Li(School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment,Engineering Research Center of Landscape Environment of Ministry of Education,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing 100083,China)

机构地区：[1]北京林业大学园林学院,北京100083 [2]花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,园林环境教育部工程研究中心,林木花卉遗传育种教育部重点实验室,北京100083

出　　处：《植物科学学报》2023年第3期370-379,共10页Plant Science Journal

基　　金：北京市共建项目专项(2019GJ-03);北京园林绿化增彩延绿科技创新工程——北京园林植物高效繁殖与栽培养护技术研究项目(2019-KJC-02-10)。

摘　　要：穗花牡荆(Vitex agnus-castus L.)是观赏价值极高的夏秋开花植物,其种子繁殖困难。本研究利用石蜡切片技术,观察穗花牡荆的种实结构,同时应用多种催芽技术,探求穗花牡荆最佳的萌发方式。结果显示,穗花牡荆具有坚硬的内果皮,且种实败育率较高。实验的催芽方法中,浓硫酸浸种20 min后再用600 mg/L赤霉素浸种24 h的组合效果最好,能够显著将发芽势、发芽率和发芽指数分别提高到10%、14.44%和1.09。Vitex agnus-castus L.is a highly ornamental and important summer and autumn flowering plant used to enrich urban landscapes.However,there is a serious shortage of seedlings in the market and seed propagation is difficult.In this paper,we used paraffin sectioning to observe the fruit and seed structure of V.agnus-castus and applied various germination techniques to determine the most effective method for seed germination.Results showed that V.agnus-castus possesses a hard endocarp and exhibits a high seed abortion rate.Among the various germination methods,treating the fruit with concentrated sulphuric acid for 20 min followed by soaking in 600 mg/L GA3 for 24 h proved to be the most efficient,leading to a substantial increase in germination potential,germination rate,and germination index to 10%,14.44%,and 1.09,respectively.

关 键 词：穗花牡荆 种实结构 石蜡切片 催芽 

分 类 号：Q945[生物学—植物学]