基于SSR分子标记的裸花紫珠种质资源遗传多样性分析及DNA指纹图谱构建  

Genetic diversity analysis and DNA fingerprints construction of Callicarpa nudiflora germplasm resources based on SSR markers

作　　者：张红瑞[1] 李鑫 陈振夏[2] 胡文斌[2] 李伟 黄梅[2] 于福来[2] 杨林立 ZHANG Hong-rui;LI Xin;CHEN Zhen-xia;HU Wen-bin;LI Wei;HUANG Mei;YU Fu-lai;YANG Lin-li(College of Agriculture,Henan Agricultural University,Zhengzhou 450046,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Cultivation of Herb Medicine(Haikou),Ministry of Agriculture and Rural Affairs/Hainan Provincial Engineering Research Center for Tropical medicinal plants,Haikou 571101,China;Hainan Jiuzhitang Pharmaceutical Co.,Ltd.,Haikou 570311,China;Henan Province Hospital of Traditional Chinese Medicine,Zhengzhou 450053,China)

机构地区：[1]河南农业大学农学院,河南郑州450046 [2]中国热带农业科学院热带作物品种资源研究所农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心,海南海口571101 [3]海南九芝堂药业有限公司,海南海口570311 [4]河南省中医院,河南郑州450053

出　　处：《中草药》2023年第12期3971-3982,共12页Chinese Traditional and Herbal Drugs

基　　金：海南省重大科技计划项目(ZDKJ2021001);海南省自然科学基金项目(322QN393)。

摘　　要：目的 研究裸花紫珠Callicarpa nudiflora种质资源遗传多样性,为其种质资源鉴定及优异种质筛选提供依据。方法 采用SSR分子标记技术,探究103份裸花紫珠种质遗传多样性,基于遗传距离进行UPGMA聚类分析,以SSR扩增条带为基础建立供试材料扩增条带DNA指纹图谱。结果 14对引物共扩增出92个等位基因(number of alleles,Na),有效等位基因(effective number of alleles,Ne)占比39.37%。平均多态性信息含量(polymorphism information content,PIC)为0.468 2,6对引物具有高度多态性(PIC>0.5),6对具有中度多态性(0.25<PIC<0.5)。平均Nei多样性指数(Nei’s gene diversity index,H)和Shannon指数(Shannon’s information index,Ⅰ)分别为1.039 0、0.505 1,表现出较高的遗传多样性水平。聚类分析将材料分为2大类:类群Ⅰ包括2份种质;类群Ⅱ包含101份种质,并分为2个亚类。主坐标分析将材料分为3个类群,与聚类结果基本一致。构建的指纹图谱通过引物组合能较好地将种质进行区分。结论 103份裸花紫珠种质具有丰富的遗传多样性,并成功利用14对SSR引物构建裸花紫珠种质DNA指纹图谱,可为裸花紫珠种质鉴定、亲缘关系以及分子辅助育种提供科学依据。Objective To study the genetic diversity and genetic relationship of Luohuazizhu(Callicarpa nudiflora Hook.et Arn.)germplasm resources,provide the basis for identification of germplasm resources and screening of excellent germplasm resources.Methods Genetic diversity of 103 germplasm resources was explored by SSR molecular marker technique,UPGMA cluster analysis was carried out based on genetic distance,and DNA fingerprints of amplified bands of tested materials were established based on SSR amplified bands.Results A total of 92 alleles(Na)were amplified by 14 pairs of primers,effective number of alleles(Ne)accounted for 39.37%.The average polymorphism information content(PIC)was 0.4682,six pairs of primers were highly polymorphic(PIC>0.5)and six pairs were moderately polymorphic(0.25<PIC<0.5).The average Nei’s gene diversity index(H)and Shannon’s information index(I)were 1.0390 and 0.5051,showing a high level of genetic diversity.Cluster analysis divided the materials into two categories:Group I included two germplasm;Group II contains 101 germplasm resources and is divided into two subclasses.Principal coordinate analysis divided the materials into three groups,which were basically consistent with the clustering results.The constructed fingerprint can distinguish germplasm by primer combination.Conclusion A total of 103 germplasms have rich genetic diversity.The DNA fingerprint of C.nudiflora germplasms was successfully constructed by 14 pairs of SSR primers.The results can provide scientific basis for germplasm identification,genetic relationship and molecular assisted breeding of C.nudiflora germplasms.

关 键 词：裸花紫珠 SSR分子标记 遗传多样性 遗传距离 聚类分析 主坐标分析 DNA指纹图谱 

分 类 号：R286.12[医药卫生—中药学]