横经席药材的生药学研究及有效成分测定  

Pharmacognostic identification and determination of active ingredient of Calophylli Herba

作　　者：林雀跃 张颖婷 梁柳春 姚力 钟小清[2] 唐倩 罗轶 LIN Que-yue;ZHANG Ying-ting;LIANG Liu-chun;YAO Li;ZHONG Xiao-qing;TANG Qian;LUO Yi(Guangxi Institute for Food and Drug Control,Nanning 530021,China;Gulin Sanjin Pharmaceutical Co.,Ltd.,Guilin 541004,China)

机构地区：[1]广西壮族自治区食品药品检验所,广西南宁530021 [2]桂林三金药业股份有限公司,广西桂林541004

出　　处：《中草药》2023年第12期4012-4018,共7页Chinese Traditional and Herbal Drugs

基　　金：国家药监局药品注册司专项“特色民族药检验方法的示范性研究”(NIFDC-TCM2021-047-MZY009);广西科技重大专项“80种桂产特色中药材配方颗粒制备工艺与质量标准及产品开发研究”(桂科AA22096019)。

摘　　要：目的对广西民族药材横经席CalophylliHerba进行基原调查和生药学研究,并对有效成分进行测定。方法通过查阅文献厘清横经席的历史使用沿革及植物分布情况,野外实地调研横经席的植物基原,采集并制作腊叶标本,通过药材性状、显微特征等方法进行生药学研究,采用岛津液相色谱仪Nexera LC-40,岛津Shim-pack GIST C_(18)-AQ HP色谱柱(100 mm×2.1 mm,1.9μm),乙腈-0.4%甲酸水为流动相,体积流量0.3 mL/min,梯度洗脱,柱温35℃,PDA检测器,检测波长275nm,建立横经席药材中穗花杉双黄酮和异棘苷的含量测定方法。结果横经席的野外资源日益匮乏,市场上常见东南亚进口的红厚壳属植物与横经席混用,采用建立的方法能够有效地鉴别横经席药材。所建立的含量测定方法稳定性和重复性良好,线性范围宽(5.360~379.800μg/mL)、线性较好(R^(2)>0.999),平均回收率在98.3%~99.0%。结论为完善横经席药材质量标准和药合理应用提供依据。Objective To study present resource situation and establish methods for the pharmacognostic identification and determination of the active ingredient of Hengjingxi(Calophylli Herba).Methods To investigate the original plant sources and the folk use Calophylli Herba by referring to the herbal literature.The original plants of Calophylli Herba were surveyed and the original plant specimens were collected through field investigation.The pharmacognosy of Calophylli Herba was studied by morphologic characteristics,character identification,microscopic identifications and TLC identification.A UPLC-UV method for simultaneous determination amentoflavone and isoapetalic acid in Calophylli Herba was established by using Shimadzu Shim-pack GIST C_(18)-AQ HP column(100 mm×2.1 mm,1.9μm),acetonitrile-0.4%formic acid water as mobile phase,gradient elution with the flow rate of 0.3 mL/min,the column temperature was 35℃,and the detection wavelength of PDA detector was 275 nm.Result The wild resource of Calophylli Herba had reduced gradually.The other plants of genus Calophyllum which imported from Southeast Asia were mixed with Calophylli Herba.The established method can be used for the identification of Calophylli Herba.The two compounds have good separation,stability and repeatability,with wide linear range(5.360—379.8μg/mL)and good linearity(R^(2)>0.999).The average recovery was 98.3%—99.0%.Conclusion The pharmacognostic identification can provide a theoretical basis for quality control and medical material supply of Calophylli Herba for pharmaceutical factory.

关 键 词：横经席 基原调查 性状鉴别 显微鉴别 穗花杉双黄酮 异棘苷 UPLC 

分 类 号：R286.2[医药卫生—中药学]