鲤CISH基因克隆鉴定与组织特异性表达分析  

Molecular identification of cytokine inducible SH-2 containing protein(CISH)gene from common carp Cyprinus carpio and its expression pattern

作　　者：杨慧 周杰 高谦 刘明丽[3] 翟万营[3] 陈良标 谢海侠[1,2] YANG Hui;ZHOU Jie;GAO Qian;LIU Mingli;ZHAI Wanying;CHEN Liangbiao;XIE Haixia(State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072 [2]中国科学院大学,北京100049 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2023年第3期491-499,共9页Journal of Shanghai Ocean University

基　　金：国家自然科学基金面上项目(31272666);国家科技支撑计划课题(2012BAD25B0202)。

摘　　要：克隆得到长度为1685 bp的鲤(Cyprinus carpio)CISH基因(Cytokine inducible SH-2 containing protein,CISH)的cDNA全长序列,开放阅读框(ORF)长666 bp,编码221个氨基酸,预测蛋白质分子量为24.91 ku。鲤CISH基因具有典型的SOCS家族结构特征,含有N区、中央SH2结构域和C端SOCS box结构域。系统进化分析表明,鲤CISH与同属鲤科的草鱼(Ctenopharyngodon idella)和斑马鱼(Danio rerio)的亲缘关系最近。鲤CISH基因的mRNA表达水平在血液中最高,肝脏中最低。嗜水气单胞菌(Aeromonas hydrophila)刺激后,鲤CISH基因在血液中表达量在12~24 h出现显著上升,在第48小时恢复正常水平,暗示鲤CISH基因在抵抗细菌感染过程中起作用。Janus kinase-signal transducers and activators of transcription(JAK-STAT)signaling pathway is one of the most important ubiquitous cell signaling in animals.In vertebrates,it is mainly involved in regulating the expression and secretion of many cytokines.The members of the suppressor of cytokine signaling(SOCS)family are the important JAK-STAT pathway inhibitory factors.Cytokine-induced SH-2 containing protein(CISH)is one known family members of SOCS,with its main function involving in regulating the signaling of interleukin-2.In this study,the full-length cDNA sequence of Cyprinus carpio CISH with the length of 1685 bp was cloned.The open reading frame(ORF)was 666 bp in length and encoded 221 amino acids.The molecular weight of the deduced protein was 24.91 kD.C.carpio CISH possesses typical SOCS family structure,with three functional domains including N region,central SH2 domain and C terminal SOCS box domain,the latter two being conservatively present in CISH proteins of other bony fish.The phylogenetic analysis showed that the common carp CISH was the closest to CISHs of grass carp and zebrafish,which also belong to Cyprinidae.Our results showed that the mRNA expression level of C.carpio CISH in blood was the highest,and the lowest expression level in liver.After being stimulated by Aeromonas hydrophila,the expression of C.carpio CISH in blood was up-regulated significantly at 24 h,and returned to the normal level at 48 h,suggesting that it may play an important role in the process of the resistance to bacterial infection.

关 键 词：鲤 细胞因子信号转导抑制因子 CISH CDNA全长 组织表达 

分 类 号：S917[农业科学—水产科学]