黄化病槟榔园中8种植物植原体分子检测及其遗传变异分析  

Molecular Detection and Genetic Variation of Phytoplasmas from Eight Plants in Garden of Areca Palm with Yellow Leaf Disease in China

作　　者：朱安娜 于少帅 苏莉惠 刘丽[2] 宋薇薇[2] 阎伟[2] ZHU Anna;YU Shaoshuai;SU Lihui;LIU Li;SONG Weiwei;YAN Wei(College of Forestry,Hainan University,Haikou,Hainan 570228,China;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571339,China;College of Tropical Crops,Yunnan Agricultural University,Pu’er,Yunnan 665099,China)

机构地区：[1]海南大学林学院,海南海口570228 [2]中国热带农业科学院椰子研究所,海南文昌571339 [3]云南农业大学热带作物学院,云南普洱665099

出　　处：《热带作物学报》2023年第6期1190-1202,共13页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务费专项(No.1630152022007,No.1630152022004);海南省自然科学基金高层次人才项目(No.320RC743)。

摘　　要：由植原体引起的槟榔黄化病是槟榔的一种毁灭性病害,明确发病槟榔园中植原体遗传多样性及其自然寄主种类,对于全面揭示该病害循环途径及流行规律具有重要意义。本研究对海南不同地区发病槟榔园中表现典型植原体病害症状的植物样品进行调查、采样,利用植原体通用引物进行扩增测序,揭示相关植原体株系的遗传变异规律与系统发育关系。结果表明:从6个山黄麻丛枝样品中扩增到植原体16S rRNA和secA基因片段,不同样品的2个基因序列均一致,从1个苦楝丛枝样品中扩增到16S rRNA基因片段。基于16S rRNA基因序列比对分析表明,山黄麻植原体与已报道山黄麻丛枝植原体序列相似性为100%,苦楝植原体与已报道苦楝丛枝植原体序列相似性为100%。系统发育分析表明:山黄麻丛枝植原体与16SrⅩⅩⅩⅡ组植原体株系聚于一个进化分支,苦楝丛枝植原体与16SrⅠ组植原体株系聚于一个进化分支;苦楝丛枝植原体与海南已报道槟榔黄化植原体16S rDNA序列相似性为100%。及时清理发病槟榔园中苦楝等植原体自然寄主,消除病源,切断植原体的传播途径,对于槟榔黄化病的有效防控至关重要。Areca palm yellow leaf(AYL)disease caused by phytoplasma is a devastating disease of areca palm planta-tion.It is of great significance to clarify the genetic variation of phytoplasmas and the natural hosts in the garden of areca with yellow leaf diseases for the purpose of fully reveal the circulation path and epidemic law of the disease.In this study,the plant samples showing typical disease symptoms associated with phytoplasma were investigated and sampled from the diseased areca garden distributed in different areas of Hainan provinve,China.The target sequences of phytoplasma were amplified and sequenced with universal primers,revealing genetic variation and phylogeny of the phytoplasma.The results showed that 16S rRNA and secA gene fragments specific for phytoplasma was amplified from six plant sample of Trema tomentosa,which were all identical.The 16S rRNA gene fragment of phytoplasma was ob-tained from one plant sample of Chinaberry.Sequence analysis based on the 16S rRNA gene sequences indicated that the T.tomentosa witches’-broom phytoplasma(TtWB)strains identified in the study were in 100%similarity with TtWB phytoplasma reported previously.Chinaberry witches’-broom(CWB)phytoplasma strains identified in the study were in 100%similarity with CWB phytoplasma strains reported previously.Phylogenetic analysis indicated that TtWB phyto plasma strains identified in the study were clustered into one evolutionary branch with the phytoplasmas belonging to 16SrⅩⅩⅩⅡgroup,CWB phytoplasma strain identified in the study were clustered into one evolutionary branch with the phytoplasmas belonging to 16SrⅠgroup.16S rDNA sequence similarity of phytoplasma was 100%among the strains of CWB and AYL.It is perfectly important for effective prevention and control of areca palm yellow leaf disease to timely clean up the natural hosts of phytoplasma such as chinaberry in areca diseased garden,eliminating the infec-tion source and cutting off the transmission routes of the phytoplasma.

关 键 词：植原体 槟榔黄化病 自然寄主 分子检测 遗传多样性 

分 类 号：S763.7[农业科学—森林保护学]