百子莲开花相关基因ApVIN3的克隆及表达分析  

Cloning and Expression Analysis of Flowering Related Gene ApVIN3 in Baizilian(Agapanthus praecox ssp.orientalis)

作　　者：石玉波[1] 汪成忠[2] 张琰[3] Shi Yubo;Wang Chengzhong;Zhang Yan(College of Modern Agriculture,Jiaxing Vocational and Technical College,Jiaxing,314036;College of Landscape Engineering,Suzhou Polytechinic Institute of Agriculture,Suzhou,215008;Garden Department,Shanghai Vocational Technical College of Agriculture and Forestry,Shanghai,201600)

机构地区：[1]嘉兴职业技术学院现代农业学院,嘉兴314036 [2]苏州农业职业技术学院园林工程学院,苏州215008 [3]上海农林职业技术学院园林系,上海201600

出　　处：《分子植物育种》2023年第13期4221-4228,共8页Molecular Plant Breeding

基　　金：浙江省科技厅项目(2021C35121);嘉兴市科技特派员项目(K2022C019)共同资助。

摘　　要：研究以百子莲(Agapanthus praecox ssp.orientalis)茎尖组织为材料,利用RACE(cDNA末端快速扩增)方法,获得百子莲VIN3基因c DNA全长序列,命名为ApVIN3,GenBank登录号为KJ472789。序列分析表明,百子莲ApVIN3基因cDNA全长1943 bp,其中开放阅读框为1680 bp,编码559个氨基酸,5'非编码区(5'UTR)和3'非编码区(3'UTR)分别为88和175 bp。ApVIN3编码一个具有C4HC3(cys4-His-cys3)保守序列结构的PHD-finger蛋白,与二穗短柄草(XP_003573553.1)VIN3蛋白有很高的相似性。实时荧光定量分析表明,ApVIN3在百子莲茎尖中的表达量受温度调控的影响,其表达量随着低温时间的延长逐渐上升。ApVIN3可能在百子莲成花诱导过程中发挥着重要的作用。A core fragment highly homologous with VERNALIZATION INSENSITIVE 3(VIN3)was obtained on the basis of preliminary Agapanthus praecox ssp.orientalis transcriptome sequencing results.The full-length cDNA sequence of VIN3 gene in A.praecox ssp.orientalis was obtained by RACE method,which was named ApVIN3 with the accession number of KJ472789.The results of sequence analysis showed that the full length of ApVIN3 cDNA was 1943 bp,which contained an open reading frame of 1680 bp,559 encoding amino acids,untranslated region(UTR)5'and 3'with the length of 88 and 175 bp,respectively.ApVIN3 encoding a PHD-finger protein with a conserved sequence structure of C4HC3(cys4-His-cys3),which was highly similar to the VIN3 protein of Brachypodium distachyum(L.)Beauv(XP_003573553.1).Quantitative Real-time PCR showed that the expression of Ap-VIN3 gene in stem tip of A.praecox ssp.orientalis was affected by temperature regulation,and the expression lev el increased gradually with the extension of low temperature time.This gene may play an important role in the floral induction process of A.praecox ssp.orientalis,and its function and regulation mechanism can be studied deeply.

关 键 词：百子莲 ApVIN3基因 克隆 基因表达 

分 类 号：S682.19[农业科学—观赏园艺]