两种转基因方法对转GmXTH抗旱基因大豆根系的影响  

The Effects on the Soybean Root System of Transgenic GmXTH Gene by Two Transgenic Methods

作　　者：张瀚竹 张野[1] 杜叶垚 宋阳[1] Zhang Hanzhu;Zhang Ye;Du Yeyao;Song Yang(College of Agriculture,Jilin Agricultural University,Changchun,130118)

机构地区：[1]吉林农业大学农学院,长春130118

出　　处：《分子植物育种》2023年第13期4278-4286,共9页Molecular Plant Breeding

基　　金：吉林省科技发展计划-优秀青年人才基金项目(20190103120JH);第四批吉林省青年科技人才托举工程项目(QT202020)共同资助。

摘　　要：为分析干旱胁迫下,两种不同方法得到的转基因大豆抗旱性差异,本试验选用农杆菌介导法得到的转GmXTH基因大豆品系OEA1、OEA2、OEA3、OEA4和花粉管通道转化得到的转基因大豆品系OET1、OET2、OET3、OET4和对照M18为试验材料,设置不同浓度梯度的PEG-6000模拟干旱胁迫的实验环境,鉴定苗期抗旱能力的差异。结果表明,随着干旱胁迫加重,两种不同转基因方法得到的转基因株系间叶片含水量、POD活性、SOD活性、丙二醛含量之间无显著差异,转基因大豆品系的抗旱能力比对照M18有显著提高,但不同转基因方法得到的大豆品系之间的抗旱能力无差异。In order to analyze the difference of drought resistance of transgenic soybean obtained by two different methods under drought stress.We selected the transgenic GmXTH soybean strains OEA1,OEA2,OEA3,OEA4 obtained by Agrobacterium-mediated method,the transgenic soybean strains OET1,OET2,OET3,OET4 obtained from pollen tube pathway transformation and control M18 to be the test materials in this study.In this experiment,PEG-6000 with different concentration gradients was set to simulate drought stress environment to identify the difference of drought resistance at seedling stage.The results showed that there were no significant differences in leaf water content,POD activity,SOD activity and malondialdehyde content between the two transgenic lines with increased drought stress.The drought resistance of transgenic soybean strains was significantly improved compared with control M18.However,there was no difference in drought resistance of soybean strains obtained by different transgenic methods.

关 键 词：大豆 根系 农杆菌介导法 花粉管通道 抗旱 

分 类 号：S565.1[农业科学—作物学]