细胞分裂素对水稻穗部性状和茎秆维管束结构的影响  

Effects of Cytokinins on Panicle Traits and Structure of Stem Vascular Bundles

作　　者：张大双 雷月 宫彦龙 李祖军 朱速松[1] Zhang Dashuang;Lei Yue;Gong Yanlong;Li Zujun;Zhu Susong(Rice Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang,550006)

机构地区：[1]贵州省农业科学院水稻研究所,贵阳550006

出　　处：《分子植物育种》2023年第13期4360-4367,共8页Molecular Plant Breeding

基　　金：贵州省农科院青年基金项目(黔农科院青年科技基金[2021]13号);中央引导地方资金项目(黔科中引地[2019]4001);贵州省水稻研究所项目(黔水稻所青年基金[2021]007号)共同资助。

摘　　要：为了明确外源细胞分裂素(cytokinins,CTK)对水稻穗部性状和茎秆维管束结构的影响,以2个粳稻品种为试验材料,分别在分蘖期(T_(1))、拔节期(T_(2))和抽穗期(T_(3))喷施零浓度(CK,0 mg/L)、低浓度(C_(1),10 mg/L)、中浓度(C_(2),15 mg/L)和高浓度(C_(3),25 mg/L)的CTK,研究其对水稻穗部性状和茎秆维管束结构的影响。结果表明,喷施不同浓度的CTK对水稻穗部性状和茎秆维管束结构影响显著。喷施不同浓度CTK后,穗长、穗粒数、二次枝梗结实率和整穗结实率、大维管束数目、小维管束数目、大维管束面积和韧皮部面积显著高于对照组,但千粒重显著降低。不同时期喷施CTK,对水稻穗粒数的影响为T_(1)>T_(3)>T_(2),对结实率影响较少,仅T_(2)时期施用CTK二次枝梗结实率和整穗结实率显著高于其他2个时期。相关分析表明,穗长和一次枝梗结实率与大维管束数和木质部面积呈极显著的正相关,及整穗结实率与大维管束数、大维管束面积和木质部面积均呈极显著的正相关。探讨施用CTK后穗部性状和茎秆维管束结构的关系,为今后在水稻生产上应用CTK调控穗部性状和茎秆维管束结构提供了实验依据。Two rice cultivars of japonica were used as test materials to study the effects of spraying cytokinins(CTK)with zero(CK,0 mg/L),lower(C1,10 mg/L),medium(C2,15 mg/L)and high(C3,25 mg/L)concentrations on panicle traits and stem vascular bundles of rice during panicle differentiation stages,which including at the tillering stage(T_(1)),the jointing stage(T_(2)),and the heading stage(T_(3))to investigate the effects of CTK on panicle traits and stem vascular bundles in rice.The results were as follows,spraying CTK with different concentrations had significant effects on panicle traits and stem vascular bundle.Compared with CK,panicle length,grain number,seed setting rate of secondary branches,the seed setting rate of whole panicle,the number of large vascular bundles,the number of small vascular bundles,large vascular bundles area and xylem area were significantly higher than that of the control,but 1000-grain weight was significantly decreased.After spraying CTK at different stage,The effect of promoting grain number was T_(1)>T_(3)>T_(2),and the effects promoting seed setting rate of sec-ondary branch and seed setting rate of whole panicle were jointing stage(T_(2))extremely significantly higher than that at other stages.Correlation analysis showed the panicle length and seed setting rate of primary branches was positively correlated with the number of large vascular bundles and xylem area.The seed setting rate of whole panicle was positively correlated with the number of larger vascular bundle,larger vascular bundle area and xylem area.To investigate the relationship between panicle traits and vascular bundle structure of stem after CTK application,and to provide theoretical reference and scientific basis for applying CTK to regulate panicle traits and vascular bundle structure of stem in rice production in the future.

关 键 词：细胞分裂素 穗部性状 维管束 粳稻 

分 类 号：S511[农业科学—作物学]