不同酶制剂组合对水稻秸秆青贮品质和体外瘤胃发酵特性的影响  

Effects of Different Enzyme Preparation Combinations on Silage Quality and in Vitro Rumen Fermentation Characteristics of Rice Straw

作　　者：周苗育 武伟成 肖定福[1] 陈东[1] 蒋润瑶 曾沅沅 魏仲珊 彭繁昌 李付强 ZHOU Miaoyu;WU Weicheng;XIAO Dingfu;CHEN Dong;JIANG Runyao;ZENG Yuanyuan;WEI Zhongshan;PENG Fanchang;LI Fuqiang(College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;Hunan Dren Grass Technology Development Co.,Ltd.,Changde 415000,China;Hunan Tianhua Industrial Co.,Ltd.,Loudi 417000,China)

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]湖南德人草业科技发展有限公司,常德415000 [3]湖南天华实业有限公司,娄底417000

出　　处：《动物营养学报》2023年第6期3856-3866,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：湖南省重点研发计划项目(2022NK2025);湖湘英才项目(2021RC3139);国家肉牛牦牛产业技术体系项目(CARS-37);湖南省草食动物产业技术体系。

摘　　要：本试验旨在探究不同酶制剂组合对水稻秸秆青贮品质和体外瘤胃发酵特性的影响。试验以水稻秸秆为青贮原料,添加不同酶制剂组合,共设4个组:对照组(CK组,不添加任何酶制剂)、C组(添加1.0 g/kg纤维素酶、木聚糖酶、β-葡聚糖酶复合酶制剂)、PL组(添加0.5 g/kg果胶酶+1.0 g/kg漆酶)、CPL组(添加1.0 g/kg纤维素酶、木聚糖酶、β-葡聚糖酶复合酶制剂+0.5 g/kg果胶酶+1.0 g/kg漆酶),每组5个重复。青贮60 d后开包取样,分析其青贮品质和体外瘤胃发酵特性。结果表明:1)水稻秸秆青贮后,PL组的干物质含量显著高于CK、C、CPL组(P<0.05);CPL组的粗脂肪含量、相对饲用价值、总可消化养分显著高于CK组(P<0.05),中性洗涤纤维和酸性洗涤纤维含量显著低于CK组(P<0.05)。2)体外发酵24 h后,C、PL、CPL组24 h累积产气量、慢速降解部分产气量和潜在产气量显著高于CK组(P<0.05),快速部分产气量显著低于CK组(P<0.05);PL、CPL组的慢速降解部分的产气速率显著高于CK、C组(P<0.05)。3)C、PL、CPL组的pH显著低于CK组(P<0.05),CPL组的氨态氮含量显著高于C、CK组(P<0.05)。4)C、PL、CPL组干物质降解率显著高于CK组(P<0.05),CPL组的粗蛋白质降解率显著高于CK组(P<0.05),CPL组的中性洗涤纤维降解率显著高于CK、PL组(P<0.05)。由此可见,添加不同酶制剂组合可以在一定程度上改善水稻秸秆青贮品质和体外瘤胃发酵特性,在生产实际中,建议以纤维素酶、木聚糖酶、β-葡聚糖酶复合酶制剂+漆酶+果胶酶的组合添加,有利于获得品质较佳的水稻秸秆青贮饲料。This experiment was designed to investigate the effects of different enzyme preparation combinations on silage quality and in vitro rumen fermentation characteristics of rice straw.The experiment used rice straw as silage material,and added different enzyme preparation combinations,four groups were established:control group(CK group,no enzyme preparation added),C group(added 1.0 g/kg cellulase,xylanase andβ⁃glu⁃canase compound enzyme preparation),PL group(added 0.5 g/kg pectinase+1.0 g/kg laccase)and CPL group(added 1.0 g/kg cellulase,xylanase andβ⁃glucanase compound enzyme preparation+0.5 g/kg pectinase+1.0 g/kg laccase),with five replicates in each group.Samples were opened after 60 days of silage,and the silage quality and in vitro rumen fermentation characteristics were analyzed.The results showed as fol⁃lows:1)the rice straw after silage,the dry matter content of PL group was significantly higher than that of CK,C and CPL groups(P<0.05);the ether extract,relative feed value and total digestible nutrients of CPL group were significantly higher than those of CK group(P<0.05),and the neutral detergent fiber and acid de⁃tergent fiber contents were significantly lower than those of CK group(P<0.05).2)After 24 h of in vitro fer⁃mentation,the 24 h cumulative gas production,gas production in the slow degradation fraction and potential gas production of C,PL and CPL groups were significantly higher than those of CK group(P<0.05),and the gas production in the fast fraction was significantly lower than that of CK group(P<0.05);the gas production rate in the slow degradation fraction of PL and CPL groups was significantly higher than that of CK and C groups(P<0.05).3)The pH of C,PL and CPL groups was significantly lower than that of CK group(P<0.05),and the ammonia nitrogen content of CPL was significantly higher than that of C and CK groups(P<0.05).4)The dry matter degradation rate of C,PL and CPL groups was significantly higher than that of CK group(P<0.05),the crude protein degradation rate of CPL group

关 键 词：水稻秸秆 酶制剂 体外瘤胃发酵 

分 类 号：S816.53[农业科学—饲料科学]