Combination of Shengmai San and Radix puerariae ameliorates depression-like symptoms in diabetic rats at the nexus of PI3K/BDNF/SYN protein expression  

作　　者：Ayaz Ahmed Guirong Zeng Mudassar Azhar Fengzhong Wang Jingru Wang Bei Fan Xinmin Liu Dejiang Jiang Qiong Wang 

机构地区：[1]Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing,China [2]Hunan Key Laboratory of Pharmacodynamics and Safety Evaluation of New Drugs&Hunan Provincial Research Center for Safety Evaluation of Drugs,Changsha,China [3]Dr.Panjwani Center for Molecular Medicine and Drug Research,International Center for Chemical and Biological Sciences,University of Karachi,Karachi,Pakistan [4]Institute of Drug Discovery Technology,Ningbo University,Ningbo,China [5]Research Center for Pharmacodynamic,Material Basis and Mechanism of Action,College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang,China [6]Sino-Portugal TCM International Cooperation Center,The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou,China

出　　处：《Animal Models and Experimental Medicine》2023年第3期211-220,共10页动物模型与实验医学（英文）

基　　金：We thank the National High-End Foreign Experts Recruitment Plan(G2022051012L);National Key Research and Development Program of China(2016YFE0131800);Science and Technology Department of Sichuan Province(2019YFH0023);the High-End Talents Recruitment Program(Liu Xinmin group)of Luzhou Municipal People's Government.

摘　　要：Background:Hyperglycemia is a characteristic feature of diabetes that often results in neuropsychological complications such as depression.Diabetic individuals are more vulnerable to experience depression compared to the normal population.Thus,novel treatment approaches are required to reduce depressive symptoms among diabetic individuals.Traditional Chinese medicines(TCMs)such as Shengmai San(SMS)and Radix puerariae(R)are usually widely used to treat ailments such as neurological com-plications since ancient time.Methods:In this study,SMS was combined with R to prepare an R-SMS formulation and screened for their antidepressant activity in diabetic rats.The antidepressant po-tential of the prepared combination was evaluated behaviorally using open field test,novelty-induced hypophagia,and forced swim test in diabetic rats with biochemical and protein expression(PI3K,BDNF[brain-derived neurotrophic factor],and SYN[pr-esynaptic vesicle protein])analysis.Results:Diabetic rats(streptozotocin,45 mg/kg)showed elevated fasting blood glu-cose(FBG)>12 mM with depressive symptoms throughout the study.Treatment with R-SMS(0.5,1.5,and 4.5 g/kg)significantly reverted depressive symptoms in diabetic rats as evinced by significantly(p<0.05)reduced immobility time with an increased tendency to eat food in a novel environment.Treatment with R-SMS also significantly increased the protein expression of PI3K,BDNF,and SYN protein,which play a crucial role in depression.Conclusion:This study showed that R-SMS formulation antagonized depressive symptoms in diabetic rats;thus,this formulation might be studied further to develop as an antidepressant.

关 键 词：BDNF DEPRESSION Shengmai San STZ induced diabetes traditional Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]