藏茵陈环烯醚萜类化合物改善油酸诱导的肝细胞脂肪变性  

Oleic Acid-Induced Hepatocyte Steatosis Improved by Iridoids of Tibetan Yin Chen

作　　者：温授惠 宋阳 何杰[1] 李刚[1] 董琦[2] 王洪伦[2] 王振华[1] WEN Shouhui;SONG Yang;HE Jie;LI Gang;DONG Qi;WANG Honglun;WANG Zhenhua(Center for Mitochondria and Healthy Aging,School of Life Sciences,Yantai University,Yantai 264005,China;Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining 810008,China)

机构地区：[1]烟台大学生命科学学院/线粒体与健康衰老研究中心,山东烟台264005 [2]中国科学院西北高原生物研究所,青海西宁810008

出　　处：《烟台大学学报（自然科学与工程版）》2023年第3期285-293,共9页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：中国科学院重点部署项目子课题(ZDRW-ZS-2020-2-3);山东省自然科学基金资助项目(ZR2019MH094)。

摘　　要：通过油酸孵育HepG2人肝癌细胞建立肝细胞脂肪变性体外模型,考察龙胆苦苷(GPS),苦龙胆酯苷(AG)和獐牙菜苷(SW)对肝细胞脂肪变性的影响及机制。结果表明,GPS、AG、SW处理可显著降低细胞内甘油三酯水平。GPS和SW可激活腺苷酸激活蛋白激酶(AMPK),上调脂质分解相关蛋白酰基辅酶A氧化酶1(ACOX1)和肉碱酰基转移酶1A(CPT1A)的表达,抑制脂质合成相关蛋白乙酰辅酶A羧化酶(ACC)、甾醇调节元件结合蛋白1c(SREBP-1c)和脂肪酸合成酶(FAS)的表达。另外,GPS、AG、SW处理均明显降低线粒体活性氧生成,提高线粒体DNA拷贝数。研究结果表明,三种藏茵陈环烯醚萜类化合物可调节脂代谢,改善肝细胞脂质沉积。To investigate the effects and mechanisms of Gentiobioside(GPS),Amarogentin(AG)and Sweroside(SW)on hepatocyte steatosis,an in vitro model of hepatocyte steatosis is established by incubating HepG2 cells with oleic acid.The results indicate that the treatment of GPS,AG and SW can significantly reduce the level of intracellular triglyceride.GPS and SW can activate adenylate-activated protein kinase(AMPK),then upregulate the expression of lipidβ-oxidation-related protein acyl-CoA oxidase 1(ACOX1)and carnitine acyltransferase 1A(CPT1A),while they also inhibite the expression of lipid synthesis-related protein acetyl-CoA carboxylase(ACC),sterol regulatory element binding protein 1c(SREBP-1c)and fatty acid synthase(FAS).Moreover,treatment with GPS,AG and SW substantially ameliorate mitochondrial functionality by reducing ROS production and increasing the mtDNA copy number.The studies confirm that the three iridoids of tibetan Yin Chen can regulate lipid metabolism and improve lipid deposition in hepatocytes.

关 键 词：肝细胞脂肪变性 环烯醚萜类化合物 藏茵陈 腺苷酸激活蛋白激酶 

分 类 号：R966[医药卫生—药理学]