不同品种厚皮甜瓜采后糖含量及糖代谢酶活性比较  

Comparison of postharvest sugar content and sugar metabolizing enzyme activities of different varieties of thick-skinned melons

作　　者：贾斌鑫 杨世英 王艳 杨朋朋 何伟忠 王成 刘峰娟 范盈盈 JIA Binxin;YANG Shiying;WANG Yan;YANG Pengpeng;HE Weizhong;WANG Cheng;LIU Fengjuan;FAN Yingying(College of Food and Pharmaceutical Sciences,Xinjiang Agricultural University,Urumqi 830052,China;Laboratory of Quality and Safety Risk Assessment for Agro-Products(Urumqi),of Ministry of Agriculture and Rural Affairs of the P.R.C./Research Institute of Agricultural Quality Standards&Testing Technology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;College of Biological and Geographical Sciences,Yili Normal University,Yining Xinjiang 835000,China)

机构地区：[1]新疆农业大学食品科学与药学学院,乌鲁木齐830052 [2]新疆农业科学院农业质量标准与检测技术研究所/农业农村部农产品质量安全风险评估实验室(乌鲁木齐)/新疆农产品质量安全重点实验室,乌鲁木齐830091 [3]伊犁师范大学生物与地理科学学院,新疆伊宁835000

出　　处：《新疆农业科学》2023年第6期1476-1484,共9页Xinjiang Agricultural Sciences

基　　金：自治区公益性科研院所基本科研业务经费项目(KY2020108,KY2021139)。

摘　　要：【目的】研究新疆不同品种甜瓜糖组分及其相关代谢酶活性差异。【方法】以9种新疆特色厚皮甜瓜为试材,提取和测定甜瓜中可溶性糖和转化酶(Ivr)、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS),分析糖组分和糖代谢相关酶相关性。【结果】不同品种甜瓜的果糖、葡萄糖和蔗糖含量存在差异,果糖、葡萄糖和蔗糖含量范围分别为2.17~3.21、1.09~2.56和0.77~5.76 g/100 g;其中果糖含量最高的是黄梦脆,葡萄糖含量最高的是俊秀,蔗糖含量最高的是早醉仙。甘甜蜜宝的蔗糖磷酸合酶和蔗糖合成酶活性最高,分别为7.5和8.7μmol/(h·g),9种成熟甜瓜中转化酶处在低活性状态。【结论】不同品种厚皮甜瓜的糖组分和糖代谢相关酶存在着不同相关性。【Objective】To compare the differences in the sugar content and their associated metabolic enzyme activity among different varieties of thick-skinned melons in Xinjiang.【Methods】9 varieties of thick-skinned melons were tested.The soluble sugar,invertase(Ivr),sucrose phosphate synthase(SPS)and sucrose synthase(SS)were extracted and determined.Meanwhile,the correlation between sugar content and their associated metabolic enzyme activity were analyzed.【Results】Results showed that significant differences exist in the contents of fructose,glucose and sucrose among different varieties of melons.Fructose,glucose and sucrose content ranged from 2.17 to 3.21 g/100 g,1.09 to 2.56 g/100 g and 0.77 to 5.76 g/100 g,respectively.Among which,Huang Meng cui has the highest fructose content,Jun xiu has the highest glucose content,and Zao zui xian has the highest sucrose content.The activities of SPS and SS of"Gan tian mi bao"were the highest,which were 7.5μmol/(h·g)and 8.7μmol/(h·g),respectively.Ivr of all the 9 melons have low activity.【Conclusion】There were different correlations between sugar content and their associated metabolic enzyme activity among different varieties of melons.

关 键 词：厚皮甜瓜 可溶性糖 转化酶 蔗糖磷酸合酶 蔗糖合成酶 

分 类 号：S652[农业科学—果树学]