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作 者:陈艳 黄璐瑶 邓昌蓉[1] 张彦君 侯全刚[1] 邵登魁[1] CHEN Yan;HUANG Luyao;DENG Changrong;ZHANG Yanjun;HOU Quangang;SHAO Dengkui(Key Laboratory of Qinghai Province Vegetable Genetics and Physiology/Qinghai University,Xining 810016,China)
机构地区:[1]青海大学农林科学院/青海省蔬菜遗传与生理重点实验室,西宁810016
出 处:《新疆农业科学》2023年第6期1492-1498,共7页Xinjiang Agricultural Sciences
基 金:青海省科学技术厅应用基础研究项目(2020-ZJ-766)。
摘 要:【目的】研究茸毛型线辣椒种质资源的抗寒性以及对低温的适应性,为资源的开发利用提供参考依据。【方法】以青海省特色品种多茸毛型辣椒青线椒2号为材料,采用低温培养箱对幼苗期植株进行低温处理,测定不同处理下超氧化物歧化酶(SOD)活性、可溶性糖含量、可溶性蛋白含量、丙二醛(MDA)含量。【结果】随着胁迫时间的延长,超氧化物歧化酶(SOD)活性、可溶性糖含量、可溶性蛋白质含量、变化趋势均为先升高后降低,丙二醛(MDA)含量变化趋势为先降低后升高再降低;随着处理温度的降低,各处理组的不同生理指标变化相对趋势趋于平缓;可溶性糖含量与可溶性蛋白含量呈极显著负相关,丙二醛含量与可溶性蛋白含量呈显著正相关。【结论】茸毛型线辣椒种质资源具有更好的抗寒性以及对低温的适应性。【Objective】To study the cold resistance and adaptability to low temperature of trichome germplasm.So as to provide a scientific reference for the development and utilization of resources in the next step.【Methods】In this experiment,the characteristic variety of Qinghai province downy linear pepper cultivar‘Qingxianjiao 2’was used as the test material.The plants were treated with low temperature at seedling stage,and the activity of superoxide dismutase(SOD),the content of soluble sugar,soluble protein content and malondialdehyde(MDA)were determined under different treatments.【Results】The results showed that with the extension of stress time,the activity of superoxide dismutase(SOD),the trend of soluble sugar,soluble protein content were firstly increased and then decreased,while the trend of malondialdehyde(MDA)content was firstly decreased and then increased and again decreased.With the decrease of treatment temperature,the changes of different physiological indexes in each treatment group tended to be gentle.Correlation analysis showed that soluble sugar content was significantly negatively correlated with soluble protein content,and malondialdehyde content was significantly positively correlated with soluble protein content.【Conclusion】Combined with the significant difference and correlation analysis of each index,the purpose is to evaluate the cold resistance of special germplasm resources through biochemical indicators,indicating that hairy germplasm resources have better cold resistance and adaptability to low temperature.
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