Chemokine platelet factor 4 accelerates peripheral nerve regeneration by regulating Schwann cell activation and axon elongation  

作　　者：Miao Gu Xiao Cheng Di Zhang Weiyan Wu Yi Cao Jianghong He 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Nantong University,Nantong,Jiangsu Province,China [2]School of Basic Medical Sciences,Hebei Key Laboratory of Nerve Injury and Repair,Chengde Medical University,Chengde,Hebei Province,China

出　　处：《Neural Regeneration Research》2024年第1期190-195,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.31730031,32130060;the National Natural Science Foundation of China,No.31971276(to JH);the Natural Science Foundation of Jiangsu Province,No.BK20202013(to XG);the Natural Science Foundation of Jiangsu Higher Education Institutions of China(Major Program),No.19KJA320005(to JH)。

摘　　要：Schwann cells in peripheral nerves react to traumatic nerve injury by attempting to grow and regenerate.Howeve r,it is unclear what factors play a role in this process.In this study,we searched a GEO database and found that expression of platelet factor 4 was markedly up-regulated after sciatic nerve injury.Platelet factor is an important molecule in cell apoptosis,diffe rentiation,survival,and proliferation.Further,polymerase chain reaction and immunohistochemical staining confirmed the change in platelet factor 4 in the sciatic nerve at different time points after injury.Enzyme-linked immunosorbent assay confirmed that platelet factor 4 was secreted by Schwann cells.We also found that silencing platelet factor 4 decreased the proliferation and migration of primary cultured Schwann cells,while exogenously applied platelet factor 4 stimulated Schwann cell prolife ration and migration and neuronal axon growth.Furthermore,knocking out platelet factor 4 inhibited the prolife ration of Schwann cells in injured rat sciatic nerve.These findings suggest that Schwann cell-secreted platelet factor 4 may facilitate peripheral nerve repair and regeneration by regulating Schwann cell activation and axon growth.Thus,platelet factor 4 may be a potential therapeutic target for traumatic peripheral nerve injury.

关 键 词：axon elongation bioinformatic analysis cell migration cell proliferation dorsal root ganglia peripheral nerve regeneration peripheral nerve trauma platelet factor 4 rat sciatic nerve Schwann cells 

分 类 号：R338[医药卫生—人体生理学]