儿童原发性肾病综合征血清PLCE1、TRPC6、CD2AP、ACTN4检测的意义  

The significance of serum PLCE1,TRPC6,CD2AP,ACTN4 detection in children with primary nephrotic syndrome

作　　者：黄仁勇 刘运广[2] 黄廷读 吴伟利[1] 林娜[2] 陆壮念 杨丽娟[2] 梁立婷 韦爱伯[2] 黄月艳[2] 黄云峰[2] Huang Renyong;Liu Yunguang;Huang Tingdu;Wu Weili;Lin Na;Lu Zhuangnian;Yang Lijuan;Liang Liting;Wei Aibo;Huang Yueyan;Huang Yunfeng(Department of Pediatrics,People’s Hospital of Tiandong County,Tiandong 531500,Guangxi,China;Department of Pediatrics,The Affiliated Hospital of Youjiang Medical Uniuersity for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]广西田东县人民医院儿科,广西田东531500 [2]右江民族医学院附属医院儿科,广西百色533000

出　　处：《右江民族医学院学报》2023年第3期463-466,471,共5页Journal of Youjiang Medical University for Nationalities

摘　　要：目的探讨检测外周血磷脂酶CE1(PLCE1)、瞬时受体电位阳离子通道蛋白6(TRPC6)、CD2相关蛋白(CD2AP)、α-辅肌动蛋白4(ACTN4)在儿童原发性肾病综合征(PNS)、激素敏感型肾病综合征(SSNS)、激素耐药型肾病综合征(SRNS)、激素依耐型肾病综合(SDNS)及不同病理类型表达的临床意义。方法采集原发性肾病综合征外周血清标本,采用双抗体夹心ELISA法检测样本中目PLCE1、TRPC6、CD2AP、ACTN4表达浓度。结果PNS组、SSNS组、SRNS组、SDNS组PLCE1、TRPC6、CD2AP、ACTN4表达与健康组比较降低,差异均有统计学意义(P<0.05),SRNS组、SDNS组表达与SSNS组比较降低,差异均有统计学意义(P均<0.05),SRNS组与SDNS组比较差异均无统计学意义(P>0.05);FSGS组、IgAN组血清PLCE1、TRPC6、CD2AP、ACTN4表达与MCD组比较降低,差异均有统计学意义(P<0.05),FSGS组、IgAN组分别与MsPGN组、MPGN组比较降低,差异均有统计学意义(P<0.05),MsPGN组与MPGN组比较差异无统计学意义(P>0.05),FSGS组与IgAN组比较差异无统计学意义(P>0.05)。结论①血清中PLCE1、TRPC6、CD2AP、ACTN4作为足细胞裂孔隔膜分子、足细胞信号分子、骨架分子等正常表达,对维系肾小球滤过膜的功能完整性起关键作用,一旦表达异常降低,可能导致肾病综合征发生、对激素治疗耐药或依耐;②血清中PLCE1、TRPC6、CD2AP、ACTN4表达与肾病综合征病理类型有关;③临床上检测血清中PLCE1、TRPC6、CD2AP、ACTN4表达水平对判断是否为难治性肾病、病理类型、指导用药及预测预后具有重大意义。Objective To investigate the clinical significance of peripheral blood phospholipase CE1(PLCE1),transient receptor potential cation channel 6(TRPC6),CD2-associated protein(CD2AP),andα-actinin-4(ACTN4)expression in children with primary nephrotic syndrome(PNS),steroid-sensitive nephrotic syndrome(SSNS),steroid-resistant nephrotic syndrome(SRNS),steroid-dependent nephrotic syndrome(SDNS)and different pathological types.Methods Peripheral serum samples from children with primary nephrotic syndrome were collected and the expression levels of PLCE1,TRPC6,CD2AP,and ACTN4 were measured by double antibody sandwich enzyme-linked immunosorbent assay(ELISA).Results The expression levels of PLCE1,TRPC6,CD2AP and ACTN4 in PNS group,SSNS group,SRNS group and SDNS group were lower than those in healthy group,the differences were statistically significant(P<0.05);the expression levels in SRNS group and SDNS group were lower than those in SSNS group,the differences were statistically significant(P<0.05).There was no significant difference between SRNS group and SDNS group(P>0.05).The expression levels of serum PLCE1,TRPC6,CD2AP and ACTN4 in FSGS group and IgAN group were lower than those in MCD group,and the differences were statistically significant(P<0.05);FSGS group and IgAN group were lower than those in MsPGN group and MPGN group,respectively,and the differences were statistically significant(P<0.05).There was no significant difference between MsPGN group and MPGN group(P>0.05),and there was no significant difference between FSGS group and IgAN group(P>0.05).Conclusion①Serum PLCE1,TRPC6,CD2AP and ACTN4 are normally expressed as podocyte slit diaphragm proteins,podocyte signaling proteins and cytoskeletal proteins,which are essential for maintaining the functional integrity of glomerular filtration barrier.Abnormal reduction of their expression may lead to the development of nephrotic syndrome and resistance or dependence to hormone therapy.②The expression of serum PLCE1,TRPC6,CD2AP and ACTN4 is related to the pathologic

关 键 词：原发性肾病综合征 磷脂酶CE1 α-辅肌动蛋白4 瞬时受体电位阳离子通道蛋白6 CD2相关蛋白 

分 类 号：R692[医药卫生—泌尿科学]