Key chemical composition of walnut(Juglans regia.L)Oils generated with different processing methods and their cholesterol-lowering effects in HepG2 cells  

作　　者：Pan Gao Ruijie Liu Qingzhe Jin Xingguo Wang 

机构地区：[1]Key Laboratory for Deep Processing of Major Grain and Oil(Wuhan Polytechnic University)of Ministry of Education in China,College of Food Science and Engineering,Wuhan Polytechnic University,68 Xuefu South Road,Changqing Garden,Wuhan,430023,PR China [2]Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,National Engineering Research Center for Functional Food,School of Food Science and Technology,Jiangnan University,1800 Lihu Road,Wuxi,214122,PR China

出　　处：《Food Bioscience》2022年第1期96-104,共9页食品生物科学（英文）

基　　金：supported by the National Natural Science Foundation of China Youth Science Foundation Project(grant numbers 32001735).

摘　　要：We systematically evaluated fatty acids and triacylglycerol composition,as well as tocopherol,phytosterol,and phenolics,in walnut oil and compared the cholesterol-lowering effects of oil processed with different methods(cold pressing,roast-pressing,hexane extraction,subcritical butane extraction,and supercritical CO_(2) extraction).The different methods did not affect the lipid composition of walnut oil.The tocopherol(41.11 mg/100 g)and total phenolic content(TPC,4.26 mg/100 g)of roast-pressed walnut oil and the phytosterol contents of subcritical butane-extracted walnut oil(106.51 mg/100 g)were higher than those of other tested oils.Walnut oil significantly decreased cholesterol synthesis by downregulating the expression of HMGCR,SREBP-2,and CYP51 genes,and increased cholesterol efflux by upregulating the expression of ABCG1,thus significantly reducing total cholesterol and triacylglycerol.Phytosterols and TPC in walnut oil were responsible for lowering cholesterol;the optimal concentration of phytosterols was 10μg/mL,and that of TPC was 12.5×10^(-3)μg/mL.Through process optimization,a new processing method for walnut oil based on biological evaluation was preliminarily established.

关 键 词：Walnut oil Processing method Cholesterol lowering PHENOLIC Phytosterols Juglans regia L 

分 类 号：TS22[轻工技术与工程—粮食、油脂及植物蛋白工程]