Synthesis of symmetrical medium-and long-chain triacylglycerols rich in arachidonic acid at sn-2 position for infant formula  

作　　者：Xuan Jiang Jianhua Huang Yaru Li Ye Wen Shibin Wu Xiuli Xu Zhonghao Chao Hong Zhang Xiaoqiang Zou 

机构地区：[1]National Engineering Research Center for Functional Food,Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,National Engineering Laboratory for Cereal Fermentation Technology,State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University,1800 Lihu Road,Wuxi,214122,Jiangsu,PR China [2]Joint Laboratory of Functional Food for Healthy Body Fat Loss,Chengdu Tianyi Cuisine Nutritious Food Co.,Ltd.,360 Tianhui Road,High-tech Zone,Chengdu,Sichuan,China [3]Wilmar(Shanghai)Biotechnology Research&Development Center Co.,No.118 Gaodong Road,Pudong New District,Shanghai,200137,China

出　　处：《Food Bioscience》2022年第1期386-392,共7页食品生物科学（英文）

基　　金：supported by the National Natural Science Foundation of China(31601433);Jiangsu Province Natural Science Foundation(BK20140149).

摘　　要：Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by enzymatic alcoholysis.The MAG with 69.42%ARA at sn-2 position were obtained by solvent extraction and low temperature solvent crystallization.Secondly,the MLCT rich in ARA at sn-2 position and capric acid(CA)at sn-1,3 positions were produced by enzymatic esterification.Under the optimal conditions(MAG:CA=1:3(mol/mol),0.05 MPa vacuum,8% Lipozyme RM IM,5 h,25℃),the content of triacylglycerol was up to 93.60%.The triacylglycerol in the form of C10:0-C20:4-C10:0(including isomers)was about 40.43%.The ARA contents in the total and sn-2 fatty acid composition of the final product were 32.35%and 51.12%,respectively.MLCT rich in ARA at sn-2 position were successfully produced and the product has the potential application for functional food and infant formula.

关 键 词：Arachidonic acid sn-2 monoacylglycerol LIPASE Medium-and long-chain triacylglycerol Infant formula 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]