高脂血症性急性胰腺炎患者血清miR-372、PTEN水平变化及其意义  

Changes in levels of serum miR-372 and PTEN in patients with hyperlipidemic acute pancreatitis and their significance

作　　者：蒋梦萍 李秀芬 邬宇美[1] 尉秀清[2] JIANG Mengping;LI Xiufen;WU Yumei;WEI Xiuqing(Department of Gastroenterology,The Third People's Hospital of Shenzhen,Shenzhen 518112,China;不详)

机构地区：[1]深圳市第三人民医院消化内科,广东深圳518112 [2]中山大学附属第三医院消化内科

出　　处：《山东医药》2023年第19期9-13,共5页Shandong Medical Journal

基　　金：广东省医学科研基金项目(A2019063)。

摘　　要：目的探讨高脂血症性急性胰腺炎(HLAP)患者血清微小RNA-372(miR-372)、第10号染色体上缺失的磷酸酶和紧张素同源物(PTEN)水平变化及其意义。方法选取150例HLAP患者为HLAP组,根据病情严重程度将HLAP患者分为轻症组(n=41)、中度重症组(n=43)、重症组(n=66),根据预后分为死亡组(n=34)和存活组(n=116);同期另选取62名体检者为对照组。采用实时荧光定量PCR法检测血清miR-372,酶联免疫吸附法检测PTEN。Pear-son相关法分析HLAP患者血清miR-372与PTEN水平的相关性,多因素Logistic回归分析HLAP患者预后不良的影响因素,受试者工作特征曲线分析血清miR-372、PTEN水平对HLAP患者预后的预测价值。结果HLAP组血清miR-372水平高于对照组,PTEN水平低于对照组(P均<0.05)。轻症组、中度重症组、重症组血清miR-372水平依次升高,PTEN水平依次降低(P均<0.05)。HLAP患者血清miR-372与PTEN水平呈负相关(r=-0.729,P<0.05)。重症HLAP、住ICU时间长和C反应蛋白、miR-372水平升高为HLAP患者预后不良的独立危险因素,PTEN升高为独立保护因素[OR(95%CI)分别为4.208(1.424~12.432)、1.724(1.243~2.390)、1.030(1.010~1.050)、1.672(1.271~2.200)、0.936(0.904~0.969)]。血清miR-372、PTEN水平联合预测HLAP患者预后的曲线下面积大于二者单独预测(P均<0.05)。结论HLAP患者血清miR-372水平升高、PTEN水平降低,二者与病情严重程度和预后有关,可作为HLAP患者预后不良的预测指标。Objective To investigate the changes in levels of serum microRNA-372(miR-372)and phosphatase and tensin homologue deleted on chromosome 10(PTEN)in patients with hyperlipidemic acute pancreatitis(HLAP)and their significance.Methods Totally 150 HLAP patients were selected as the HLAP group.HLAP patients were divided into the mild(n=41),moderately severe(n=43)and severe(n=66)groups according to their severity,and into death(n=34)and survival(n=116)groups according to their prognosis;another 62 examined healthy people in the same period were selected as the control group.Serum miR-372 level was measured by quantitative real-time polymerase chain reaction and PTEN level was measured by ELISA.Pearson correlation coefficient was used to analyze the correlation between serum miR-372 and PTEN levels in HLAP patients,multi-factor Logistic regression was used to analyze the factors influencing poor prognosis of HLAP patients,and ROC curve was used to analyze the predictive value of serum miR-372 and PTEN levels for poor prognosis of HLAP patients.Results Serum miR-372 level was higher in the HLAP group than in the control group,and PTEN level was lower than that in the control group(both P<0.05).Serum miR-372 levels in the mild,moderately severe,and severe groups increased in turn,while PTEN levels decreased in turn(all P<0.05).Serum miR-372 was negatively correlated with PTEN level in HLAP patients(r=-0.729,P<0.05).Multi-factor Logistic regression analysis showed that severe HLAP,prolonged intensive care unit(ICU)time and elevated C-reactive protein and miR-372 were independent risk factors for poor prognosis in HLAP patients,and elevated PTEN was an independent protective factor[OR(95%CI):4.208(1.424 to 12.432),1.724(1.243 to 2.390),1.030(1.010 to 1.050),1.672(1.271 to 2.200),and 0.936(0.904 to 0.969)].The area under the curve of serum miR-372 combined with PTEN in predicting prognosis in HLAP patients was greater than either alone(all P<0.05).Conclusion Increased serum miR-372 level and decreased PTEN level in HLAP patie

关 键 词：高脂血症性急性胰腺炎 微小RNA-372 第10号染色体上缺失的磷酸酶和紧张素同源物 病情 预后 

分 类 号：R657.5[医药卫生—外科学]