苦参碱联合PFC方案化疗对腹水型S180小鼠生存期及P170蛋白表达的影响  

Effects of matrine combined with clinical PFC chemotherapy regimen on survival and P170 protein expression in ascites-type S180 mice

作　　者：张天泽 杨真真 孙付军[3] 刘成 王军波 王利宁 盖辉[3] ZHANG Tianze;YANG Zhenzhen;SUN Fujun;LIU Cheng;WANG Junbo;WANG Lining;GAI Hui(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;不详)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]济南市第四人民医院药学部 [3]山东省中医药研究院药理研究所 [4]乳山市人民医院肿瘤科

出　　处：《山东医药》2023年第19期37-40,共4页Shandong Medical Journal

基　　金：青岛大学医疗集团科研专项(YLJT20212020)。

摘　　要：目的探讨苦参碱联合PFC方案(顺铂+5-氟尿嘧啶+环磷酰胺)化疗对腹水型S180小鼠生存期及P170蛋白表达的影响。方法选择健康昆明小鼠80只,适应性饲养1周,随机分为模型组、PFC组、5%苦参碱+PFC组、10%苦参碱+PFC组,每组20只。各组于右侧腋窝皮下注射S180细胞构建腹水型S180小鼠模型。接种7 d后,模型组予生理盐水0.2 mL/10 g体质量灌胃,其余三组予PFC方案化疗,5%苦参碱+PFC组、10%苦参碱+PFC组在PFC方案化疗同时分别予5%、10%苦参碱溶液0.2 mL/10 g体质量灌胃,每天1次。各组均干预4周。统计各组生存时间;接种移植瘤7 d开始称量体质量,之后7 d称量1次体质量,计算7 d体质量增加情况;称量完体质量后,抽取腹水,检测腹水中P170蛋白表达及S180细胞凋亡率。结果各组接种S180细胞1周肿瘤直径约为5 mm,小鼠出现精神萎靡、行动迟缓、毛发干枯蓬松。模型组第2周开始出现死亡,至第4周时全部死亡。其余各组第3周开始陆续出现死亡。与模型组比较,各药物干预组生存时间长(P均<0.01);与PFC组比较,5%苦参碱+PFC组和10%苦参碱+PFC组生存时间长(P均<0.05);与5%苦参碱+PFC组比较,10%苦参碱+PFC组生存时间长(P<0.05)。5%苦参碱+PFC组和10%苦参碱+PFC组干预2、3、4周体质量增加均高于PFC组同期,且10%苦参碱+PFC组干预2、3、4周体质量增加均高于5%苦参碱+PFC组同期(P均<0.05)。5%苦参碱+PFC组和10%苦参碱+PFC组干预3、4周腹水中P170蛋白表达均低于PFC组同期,S180细胞凋亡率均高于PFC组同期,以10%苦参碱+PFC组干预3、4周变化更明显(P均<0.05)。结论5%、10%苦参碱联合PFC方案化疗均能显著改善腹水型S180小鼠生存期,抑制腹水中P170蛋白表达,促进腹水中S180细胞凋亡,以10%苦参碱联合PFC方案化疗效果较好。Objective To investigate the effects of the combination of matrine and the PFC chemotherapy regimen(cisplatin+5-fluorouracil+cyclophosphamide)on the survival time and expression of P170 protein in ascitic S180 mice.Methods Eighty healthy Kunming mice were selected.After 1 week of adaptive feeding,they were randomly divided in-to the model group,PFC group,5%matrine+PFC group and 10%matrine+PFC group,with 20 mice in each group.The mice models of ascites-type S180 were constructed by subcutaneous injection of S180 cells into the right axilla.Seven days after inoculation,the mice in the model group were given 0.2 mL/10 g body mass by gavage,while the mice in the other three groups were given PFC chemotherapy,and mice in the 5%matrine+PFC group and 10%matrine+PFC group were given 5%matrine solution 0.2 mL/10 g body mass by gavage,once a day,respectively.All groups were treated for 4 weeks.The survival time of each group was counted.The weight of the transplanted tumor was weighed at 7 days after inoc-ulation,and then every 7 days,and the increase of the body mass in every 7 days was calculated.After weighing,ascites were extracted to detect the expression of P170 protein and apoptosis rate of S180 cells.Results At 1 week after S180 cells were inoculated in each group,the tumor diameter was about 5 mm,and the mice showed listlessness,slow move-ment,dry and fluffy hair.The mice in the model group began to die at week 2 and all died at week 4.Death began to occur in other groups at week 3.Compared with the model group,the survival time of all drug intervention groups was significant-ly prolonged(P<0.01).Compared with PFC group,survival time of 5%matrine+PFC group and 10%matrine+PFC group was significantly prolonged(P<0.05).Compared with 5%matrine+PFC group,the survival time of 10%matrine+PFC group was significantly prolonged(P<0.05).The body mass increase of 5%matrine+PFC group and 10%matrine+PFC group at 2,3 and 4 weeks after intervention was higher than that of PFC group at the same period,and the body mass increase of

关 键 词：苦参碱 化疗 生存期 S180细胞 P170蛋白 

分 类 号：R979.1[医药卫生—药品]