氯化胆碱基低共熔溶剂优化草珊瑚总黄酮提取工艺  

Optimization and extraction of total flavonoids from Sarcandra glabra(Thunb.)Nakai by choline chloride-based deep eutectic solvents

作　　者：蒋利荣 何海燕[1,2,3,4] 覃拥灵 凌远佳[1] 莫倩涵 JIANG Lirong;HE Haiyan;QIN Yongling;LING Yuanjia;MO Qianhan(College of Chemical and Biological Engineering,Hechi University,Hechi,Guangxi 546300,China;Guangxi Key Laboratory of Sericulture and Silk Ecology&Applied Intelligent Technology,Hechi University,Hechi,Guangxi 546300,China;Guangxi Modern Sericulture and Silk Collaborative Innovation Center,Hechi University,Hechi,Guangxi 546300,China;Guangxi Colleges Key Laboratory of Exploitation and Utilization of Microbial and Botanical Resources,Hechi University,Hechi,Guangxi 546300,China)

机构地区：[1]河池学院化学与生物工程学院,广西河池546300 [2]广西蚕桑生态学与智能化技术应用重点实验室,河池学院,广西河池546300 [3]广西现代蚕桑丝绸协同创新中心,河池学院,广西河池546300 [4]微生物及植物资源开发利用广西高校重点实验室,河池学院,广西河池546300

出　　处：《中国饲料》2023年第13期22-26,共5页China Feed

基　　金：广西自然科学基金项目(2020GXNSFAA297218);广西现代蚕桑丝绸协同创新中心立项课题(2022GXCSSC05);大学生创新创业计划项目(S202210605021S)。

摘　　要：为优化草珊瑚总黄酮提取工艺,采用单因素试验探究了5种氯化胆碱基低共熔溶剂、氢键受体与氢键供体的摩尔比、提取时间、液料比、提取温度对草珊瑚总黄酮得率的影响,通过L9(34)正交试验优化提取工艺。结果表明:最优工艺为氯化胆碱/乙二醇摩尔比1:25,时间60 min,液料比30:1(mL/g),温度80℃,平均得率为17.20%,RSD为0.64%。低共熔溶剂较传统溶剂(水、醇)对草珊瑚总黄酮的提取率高,氯化胆碱/乙二醇体系低共熔溶剂是一种绿色高效的提取草珊瑚总黄酮的溶剂。Deep eutectic solvents(DES)was used to evaluate and optimize the extraction process of the total flavonoid in Sarcandra glabra(Thunb.)Nakai.First of all,5 kinds of choline chloride-based DESs were selected as potential extraction solvents,and the DES extraction effect of choline chloride/ethylene glycol was determined to be the best.Then,single-factor experiments were conducted to explore the effects of DES composition,molar ratio of hydrogen bond acceptor(HBA)to hydrogen bond donor(HBD),extraction time,and ratio of liquid to material,extraction temperature on the yield of the total flavonoid in Sarcandra glabra(Thunb.)Nakai.Based on the above experiments,the orthogonal experimental design of four factors and three levels was established.The results showed that the best extraction conditions of total flavonoids in Sarcandra glabra(Thunb.)Nakai were concluded:molar ratio of choline chloride to ethylene glycol was 1:25,extraction time was 60 min,ratio of liquid to material was 30:1(mL/g),extraction temperature was 80℃.Under these conditions,the yield of total flavonoids in Sarcandra glabra(Thunb.)Nakai was 17.20%,RSD was 0.64%.Compared with the traditional solvents(water and alcohol),the deep eutectic solvent of choline chloride/ethylene glycol system was a kind of green and efficient solvent to extract the total flavonoids of Sarcandra glabra(Thunb.)Nakai.

关 键 词：低共熔溶剂 黄酮 草珊瑚 氯化胆碱 

分 类 号：S816.7[农业科学—饲料科学]