基于组织培养的黑暗处理对番茄幼苗生长的影响  

Effects of Darkness Treatment on Growth of TomatoSeedlings Based on Tissue Culture

作　　者：罗太敏 须文[1,2,3] LUO Taimin;XU Wen(College of Agriculture,Guizhou University,Guiyang,Guizhou 550025;Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province,Guiyang,Guizhou 550025;Institute of Vegetable Research,Guizhou University,Guiyang,Guizhou 550025)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州省高等学校设施蔬菜工程研究中心,贵州贵阳550025 [3]贵州大学蔬菜研究院,贵州贵阳550025

出　　处：《北方园艺》2023年第10期46-51,共6页Northern Horticulture

基　　金：国家自然科学基金资助项目(31760594);贵州省“千”层次人才培养资助项目(贵大“千”层次[2018]02号)。

摘　　要：以番茄(Lycopersicon esculentum)幼苗为试材,采用组织培养方法,研究黑暗处理对番茄幼苗表型、生长指标和自噬作用相关基因SlATG8f表达的影响,以期为进一步研究黑暗胁迫下自噬作用相关基因的表达调控机制提供参考依据。结果表明:黑暗环境条件下番茄幼苗下胚轴显著伸长,叶柄伸长变缓,叶绿素合成减少,对幼苗的根鲜质量无显著影响,但是导致植物地上部生长受到抑制;叶绿素含量下降是黑暗诱导的叶片衰老最明显的表现,在3~5 d的黑暗处理过程中,自噬相关基因SlATG8f对相对早期的黑暗处理有明显响应。Taking tomato(Lycopersicon esculentum)seedlings as test materials,tissue culture was used,the effects of darkness treatment on the phenotype,growth index and expression of autophagyrelated gene SlATG8fin tissue culture were studied,in order to provide reference for further study on the expression and regulation mechanism of autophagy-related genes under darkness stress.The results showed that the hypocotyls of tomato seedlings elongated significantly,the petioles elongated slowly,and the synthesis of chlorophyll decreased under dark condition.There was no significant effect on the root fresh weight of tomato seedlings,but the growth of shoot was inhibited.The decline of chlorophyll content was the most obvious manifestation of dark-induced senescence,and the autophagy-related gene SlATG8f had a significant response to the relatively early dark treatment during the 3-5days dark treatment.

关 键 词：番茄幼苗 黑暗处理 幼苗生长 自噬相关基因 ATG8f 

分 类 号：S641.2[农业科学—蔬菜学]