芍药GPPS基因克隆及生物信息学分析  

Cloning and Bioinformatic Analysis of Geranyl DiphosphateGene FromPaeonia lactiflora Pall.

作　　者：高凯 左永丽 卫志鹏 王占营 吴疆[4] 范丙友[2] GAO Kai;ZUO Yongli;WEI Zhipeng;WANG Zhanying;WU Jiang;FAN Bingyou(Luoyang Municipal Academy of Agricultural and Forestry Sciences/Gene Development Engineering Technology Center of Tree Peony of Henan Province/Zhongyuan Scholar Workstation,Luoyang,Henan 471023;College of Agriculture,Henan University of Science and Technology,Luoyang,Henan 471003;Luoyang Lyuzhu Horticulture Co.Ltd.,Luoyang,Henan 471131;School of Information Engineering,Yulin University,Yulin,Shaanxi 719000)

机构地区：[1]洛阳市农林科学院,河南省牡丹基因开发工程技术中心,中原学者工作站,河南洛阳471023 [2]河南科技大学农学院,河南洛阳471003 [3]洛阳绿珠园艺有限公司,河南洛阳471131 [4]榆林学院信息工程学院,陕西榆林719000

出　　处：《北方园艺》2023年第10期73-80,共8页Northern Horticulture

摘　　要：以芍药根为试材,应用RT-PCR技术克隆芍药GPPS基因并对其进行综合生物信息学分析,研究了该基因在芍药萜类化合物合成途径中的功能,以期为GPPS基因的功能及其调控芍药萜类物质代谢提供参考依据。结果表明:芍药GPPS基因cDNA全长1 557 bp, GenBank登录号为KP645373,编码421个氨基酸,分子量为46.31 kDa,等电点为7.63,为不含跨膜结构域和信号肽位点、定位于细胞质的稳定疏水蛋白;BlastP分析表明芍药GPPS蛋白质与印楝GPPS相似性最高,达78.07%;蛋白质多序列比对分析结果表明GPPS蛋白质的氨基末端高度变异;分子系统发育分析表明芍药GPPS与其它植物GPPS亲缘关系相对较远。The full length of GPPS was cloned from Paeonia lactiflora with its root as material by RT-PCR technique and its sequence was analyzed by bioinformatics,in order to lay a foundation for further study on the function of PlGPPSin the biosynthetic pathway of terpenoids.The results showed that the full length cDNA of GPPS was 1557bp(GenBank accession KP645373),which encoded 421amino acids.The molecular weight of GPPS protein was 46.31kDa,and its pI was 7.63.Bioinformatic analysis indicated that GPPS,located in plasma,was a stable hydrophobic protein without transmembrane domain or signal peptide.BlastP analysis demonstrated that that GPPS had highly similarity with GPPS fromAzadirachta indica.Multiple protein sequence analysis showed that the N-terminus was highly variable.The phylogenetic tree analysis revealed that the genetic relationship of GPPS with those from other related plants was relatively distant.

关 键 词：芍药 GPPS 基因克隆 萜类化合物 生物信息学分析 

分 类 号：S682.12[农业科学—观赏园艺]